Adeno-associated viral vector transduction of green fluorescent protein in kidney: effect of unilateral ureteric obstruction

BJU International
Keiichi ItoDiane Felsen

Abstract

To evaluate adeno-associated virus (AAV) mediated renal gene transfer, by examining the localization and time course of gene expression in the kidneys of mice with unilateral ureteric obstruction (UUO) and controls. AAV is a replication-defective virus that has the potential to deliver genes into the kidney to improve renal damage after UUO. An AAV vector carrying a green fluorescent protein (GFP) reporter gene (rAAV-GFP) was used. In control mice, GFP expression was evaluated at 4, 7, 14 and 28 days after intrapelvic injection of rAAV or phosphate-buffered saline (PBS). In mice with UUO, the left ureter was obstructed, and 24 h later either rAAV or PBS was injected; GFP expression was evaluated 4, 7 and 14 days later by direct fluorescence. In the control mice, at least 7 days was required to detect GFP expression, whereas after UUO, GFP expression was already evident at 4 days after injection. GFP was localized mainly to the medullary tubules. This study shows successful transduction of GFP into mouse kidney using an AAV vector; GFP was expressed sooner in UUO kidneys than in the controls. These results show the feasibility of using AAV to transduce GFP into the obstructed kidney, and suggest that it might be useful in transd...Continue Reading

References

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Mar 23, 2004·Journal of the American Society of Nephrology : JASN·Takashi WadaHitoshi Yokoyama
Sep 17, 2004·Human Cell·Keiichi ItoDiane Felsen

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Citations

Jul 7, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xiaoyan LuoDwight D Koeberl
Dec 12, 2012·Clinical and Experimental Pharmacology & Physiology·Yan F QiMohan K Raizada
Apr 24, 2009·Research in Veterinary Science·Masao MiyazakiAkemi Suzuki
Jul 7, 2018·Journal of the American Society of Nephrology : JASN·Yoichiro IkedaBenjamin D Humphreys
Sep 5, 2019·Clinical and Experimental Nephrology·Suguru SaitoMasahiko Kuroda
Apr 24, 2020·Molecular Diagnosis & Therapy·Jeffrey D Rubin, Michael A Barry
Aug 10, 2019·Physiological Genomics·Lori Davis, Frank Park

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