Adeno-associated virus-mediated delivery of a mutant endostatin in combination with carboplatin treatment inhibits orthotopic growth of ovarian cancer and improves long-term survival.

Cancer Research
I V SubramanianSundaram Ramakrishnan

Abstract

A human ovarian cancer cell line, which migrates to mouse ovaries and establishes peritoneal carcinomatosis, was used to evaluate the cooperative effect of an antiangiogenic gene therapy combined with chemotherapy. The ovarian carcinoma cell line MA148 was genetically modified by "Sleeping Beauty" transposon-mediated delivery of DsRed2 fluorescent protein. Stable, high-level expression of DsRed protein enabled in vivo imaging of peritoneal dissemination of ovarian cancer. Both external and internal imaging, along with histopathology, showed migration of i.p. injected human ovarian cancer cell line to mouse ovaries. Using this model, we evaluated the effect of adeno-associated virus (AAV)-mediated expression of a mutant endostatin either alone or in combination with carboplatin treatment. A single i.m. injection of recombinant AAV (rAAV)-mutant human endostatin with P125A substitution (P125A-endostatin) showed sustained expression of mutant endostatin. Antiangiogenic gene therapy inhibited orthotopic growth of ovarian cancer and resulted in 33% long-term tumor-free survival. A single cycle of carboplatin treatment combined with mutant endostatin gene therapy resulted in 60% of the animals remaining tumor free for >200 days, whic...Continue Reading

References

Dec 15, 1988·Biochemical Pharmacology·G M LaiT C Hamilton
Jun 1, 1996·European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·S L ParsonsR J Steele
Apr 1, 1998·Journal of the National Cancer Institute·J YonedaI J Fidler
Jan 23, 1999·Obstetrics and Gynecology·K A OrielP L Remington
Jan 6, 2001·Baillière's Best Practice & Research. Clinical Obstetrics & Gynaecology·M R BrownE C Kohn
Oct 24, 2001·In Vitro Cellular & Developmental Biology. Animal·E H LeungN Auersperg
Jan 5, 2002·Expert Opinion on Investigational Drugs·M Harries, S B Kaye
Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Alfonso CalvoJeffrey E Green
Sep 21, 2002·Lancet·Martin N Tattersall
Feb 1, 2003·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Wenyin ShiDietmar W Siemann
Feb 6, 2003·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Apr 15, 2004·British Journal of Cancer·Y Yokoyama, S Ramakrishnan
Aug 10, 2004·International Journal of Cancer. Journal International Du Cancer·Yumi Yokoyama, S Ramakrishnan
Jun 21, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jakub TolarBruce R Blazar

❮ Previous
Next ❯

Citations

Jun 11, 2011·Clinical and Experimental Medicine·Tian-bao WangWen-guang Dong
Oct 17, 2006·Current Oncology Reports·Kristopher J KimballRonald D Alvarez
Feb 19, 2010·Human Gene Therapy·Haritha SamaranayakeSeppo Ylä-Herttuala
May 5, 2011·International Journal of Molecular Sciences·Kristin L M BoylanAmy P N Skubitz
Nov 19, 2009·Journal of Cancer Research and Clinical Oncology·Jing JiangPeiling Li
Aug 31, 2007·Expert Opinion on Biological Therapy·Anna KanervaAkseli Hemminki
Jan 23, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jorge L Santiago-Ortiz, David V Schaffer
Nov 21, 2009·American Journal of Clinical Pathology·Melissa S DeRyckeAmy P N Skubitz
Oct 21, 2010·American Journal of Clinical Pathology·Melissa S DeryckeAmy P N Skubitz
Feb 20, 2007·Molecular Aspects of Medicine·Luca PersanoStefano Indraccolo
Jan 13, 2011·International Journal of Cancer. Journal International Du Cancer·Yawu JingS Ramakrishnan
Jun 1, 2013·Gynecologic Oncology·Amy P N SkubitzDaniel A Vallera
Dec 3, 2014·Cancer Letters·Jingfeng LuoXiaoming Yang
Sep 14, 2010·Journal of Ovarian Research·John D AndersenAmy Pn Skubitz
Jul 27, 2007·British Journal of Cancer·A Z DudekR P Hebbel
Jun 29, 2007·International Journal of Cancer. Journal International Du Cancer·Yumi Yokoyama, S Ramakrishnan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.