PMID: 9445062Jan 28, 1998Paper

Adeno-associated virus type 2-mediated gene transfer: correlation of tyrosine phosphorylation of the cellular single-stranded D sequence-binding protein with transgene expression in human cells in vitro and murine tissues in vivo

Journal of Virology
K QingA Srivastava

Abstract

Although the adeno-associated virus type 2 (AAV)-based vector system has gained attention as a potentially useful alternative to the more commonly used retroviral and adenoviral vectors for human gene therapy, the single-stranded nature of the viral genome, and consequently the rate-limiting second-strand viral DNA synthesis, significantly affect its transduction efficiency. We have identified a cellular tyrosine phosphoprotein, designated the single-stranded D sequence-binding protein (ssD-BP), which interacts specifically with the D sequence at the 3' end of the AAV genome and may prevent viral second-strand DNA synthesis in HeLa cells (K. Y. Qing et al., Proc. Natl. Acad. Sci. USA 94:10879-10884, 1997). In the present studies, we examined whether the phosphorylation state of the ssD-BP correlates with the ability of AAV to transduce various established and primary cells in vitro and murine tissues in vivo. The efficiencies of transduction of established human cells by a recombinant AAV vector containing the beta-galactosidase reporter gene were 293 > KB > HeLa, which did not correlate with the levels of AAV infectivity. However, the amounts of dephosphorylated ssD-BP which interacted with the minus-strand D probe were also a...Continue Reading

References

Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R M KotinK I Berns
Jan 1, 1987·Advances in Virus Research·K I Berns, R A Bohenzky
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T R FlotteB J Carter
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·D W RussellA D Miller
Dec 1, 1993·Proceedings of the Society for Experimental Biology and Medicine·C E WalshR J Samulski
Jun 1, 1996·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·P PingH K Hammond
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·P D KesslerB J Byrne
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·D D KoeberlA D Miller
Mar 1, 1997·Nature Medicine·K J FisherJ M Wilson
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·K QingA Srivastava

❮ Previous
Next ❯

Citations

Jan 5, 2001·Current Cardiology Reports·J R Smith-Arica, J S Bartlett
Jun 6, 2002·Journal of Cellular Biochemistry. Supplement·Arun Srivastava
Mar 15, 2005·Critical Reviews in Biochemistry and Molecular Biology·Daniel L RiggsDavid F Smith
Feb 24, 2012·Gene Therapy·M Nonnenmacher, T Weber
Mar 30, 2000·The Journal of Gene Medicine·R O Snyder
Feb 8, 2003·DNA and Cell Biology·Chooi May LaiP Elizabeth Rakoczy
Oct 18, 2016·Frontiers in Cellular Neuroscience·Haiyan LuZhijun Zhang
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·T M DalyM S Sands
Feb 23, 2000·Journal of Virology·J S BartlettR J Samulski
Jun 18, 2005·Human Gene Therapy·Terence R Flotte
Jan 19, 2008·Cancer Gene Therapy·L KrügerJ A Kleinschmidt
Apr 19, 2020·Human Gene Therapy·Anna C Maurer, Matthew D Weitzman
Apr 14, 2006·Human Genetics·Kenneth H Warrington, Roland W Herzog
Aug 3, 1999·Biological Chemistry·H Büeler
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·P Tattersall

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.