PMID: 9188645Jul 1, 1997Paper

Adeno-associated virus type 2-mediated transfer of ecotropic retrovirus receptor cDNA allows ecotropic retroviral transduction of established and primary human cells

Journal of Virology
K QingA Srivastava

Abstract

The cellular receptors that mediate binding and internalization of retroviruses have recently been identified. The concentration and accessibility of these receptors are critical determinants in accomplishing successful gene transfer with retrovirus-based vectors. Murine retroviruses containing ecotropic glycoproteins do not infect human cells since human cells do not express the receptor that binds the ecotropic glycoproteins. To enable human cells to become permissive for ecotropic retrovirus-mediated gene transfer, we have developed a recombinant adeno-associated virus type 2 (AAV) vector containing ecotropic retroviral receptor (ecoR) cDNA under the control of the Rous sarcoma virus (RSV) long terminal repeat (LTR) promoter (vRSVp-ecoR). Established human cell lines, such as HeLa and KB, known to be nonpermissive for murine ecotropic retroviruses, became permissive for infection by a retroviral vector containing a bacterial gene for resistance to neomycin (RV-Neo(r)), with a transduction efficiency of up to 47%, following transduction with vRSVp-ecoR, as determined by the development of colonies that were resistant to the drug G418, a neomycin analog. No G418-resistant colonies were present in cultures infected with either ...Continue Reading

References

Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R M KotinK I Berns
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C H SrivastavaA Srivastava
May 1, 1987·Journal of Virology·D ArmentanoE Gilboa
Jan 1, 1987·Advances in Virus Research·K I Berns, R A Bohenzky
Feb 1, 1983·Journal of Virology·A SrivastavaK I Berns
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·M P KavanaughA D Miller
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·M A KayA R Thompson

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Citations

Nov 26, 2008·Acta Pharmacologica Sinica·Wen-fang Shi, Jeffrey S Bartlett
Apr 12, 2012·Molecular Biotechnology·Rosalinda MadonnaRaffaele De Caterina
Apr 10, 2019·Gene Therapy·Bradley A HamiltonJoseph Zabner
Aug 16, 2001·International Journal of Hematology·Noriyasu SakaiTakashi Shimada
Jun 7, 2000·The Journal of Clinical Investigation·D DuanJ F Engelhardt
Sep 2, 1998·Immunologic Research·C M Seroogy, C G Fathman
Sep 26, 2006·Nucleic Acids Research·Philipp KochOliver Brüstle
Jul 27, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·H StecherA Lieber

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