Adeno-sh-beta-catenin abolishes ischemic preconditioning-mediated cardioprotection by downregulation of its target genes VEGF, Bcl-2, and survivin in ischemic rat myocardium.

Antioxidants & Redox Signaling
Mahesh ThirunavukkarasuNilanjana Maulik

Abstract

beta-Catenin, the downstream target of glycogen synthase kinase-3beta (GSK-3beta), plays a vital role in ischemic preconditioning (IP)-mediated cardioprotection. In the present study, we investigated the mechanism of IP-mediated cardioprotection through suppression of beta-catenin expression by intramyocardial injection of adeno-sh-RNA against beta-catenin (BCT) (4 x 10(8) pfu). Adeno-LacZ (LZ) was used as control. The rats were randomized into (a) LZ + ischemia-reperfusion (IR); (b) LZIPIR; (c) BCTIR; and (d) BCTIPIR. Isolated hearts from each group were subjected to 30 min of I followed by 2 h of R. Both IPIR group hearts were subjected to IP (5 min I + 10 min R; four cycles) before IR. Significant reduction in left ventricular functional recovery (78 vs. 88 mm Hg), dp/dt(max) (1,802 vs. 2,189 mm Hg/sec), and aortic flow (4 vs. 9 ml/min) was observed in BCTIPIR compared with LZIPIR at 120 min of reperfusion. Increased infarct size (42 vs. 24%) and apoptotic cardiomyocytes (122 vs. 58 counts/60 HPF) were observed in BCTIPIR compared with LZIPIR. Realtime PCR and Western blot analysis showed significant downregulation in mRNA and protein expression of VEGF, Bcl-2, and survivin in BCTIPIR compared with LZIPIR. These findings ind...Continue Reading

References

Jan 1, 1994·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·A M AlkhulaifiW B Pugsley
Jul 1, 1997·The EMBO Journal·H AberleR Kemler
Jun 26, 1998·Current Opinion in Cell Biology·M Bienz
Feb 9, 2000·The Journal of Cell Biology·J HuelskenW Birchmeier
Jul 27, 2000·The Journal of Biological Chemistry·V W DingF McCormick
Sep 29, 2000·Circulation Research·D M Yellon, A Dana
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zhong-Hua GaoDavid M Virshup
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Guangchao SuiYang Shi
May 11, 2002·Journal of Cellular and Molecular Medicine·Nilanjana Maulik, D K Das
Aug 9, 2002·The Journal of Biological Chemistry·Hyo-Soo KimKenneth Walsh
Oct 19, 2002·Circulation Research·Randy A Hall, Robert J Lefkowitz
Mar 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Silvia MarettoStefano Piccolo
Apr 15, 2004·Journal of Molecular and Cellular Cardiology·Shoji FukudaNilanjana Maulik
Apr 15, 2005·Nature·Tannishtha Reya, Hans Clevers
May 12, 2005·Trends in Cardiovascular Medicine·Derek J HausenloyDerek M Yellon
Jul 2, 2005·Methods in Molecular Biology·Guangchao Sui, Yang Shi
Nov 30, 2006·The Journal of Pharmacology and Experimental Therapeutics·Suresh Varma PenumathsaNilanjana Maulik
Dec 26, 2006·Journal of Molecular and Cellular Cardiology·Suresh Varma PenumathsaNilanjana Maulik
Jul 17, 2007·Biochimica Et Biophysica Acta·Andrew P HalestrapIgor Khaliulin

❮ Previous
Next ❯

Citations

Nov 1, 2011·Functional & Integrative Genomics·Mahesh ThirunavukkarasuNilanjana Maulik
Jun 8, 2012·Hepatobiliary & Pancreatic Diseases International : HBPD INT·Yong-Xiang XiaXue-Hao Wang
May 29, 2009·Clinical and Experimental Pharmacology & Physiology·Yu-Ping WangZhi-Min Du
Apr 29, 2014·European Journal of Pharmacology·Oomaidurai SenthamizhselvanBoobalan Raja
Sep 20, 2015·Journal of Molecular Endocrinology·Ying YingShiwei Li
Aug 4, 2012·Environmental Toxicology·Piyajit WatcharasitJutamaad Satayavivad
Nov 21, 2015·Molecular Cancer·C SanhuezaA F G Quest
Apr 14, 2012·Journal of Pediatric Surgery·Jae-Won LeeJin Soo Han
Feb 22, 2017·Journal of Molecular Endocrinology·Hui WangJingang Zheng
Sep 24, 2020·Oxidative Medicine and Cellular Longevity·Lan ZhaoShaoheng Zhang
Aug 30, 2019·Experimental Cell Research·Neetu SinghKashif Hanif

❮ Previous
Next ❯

Datasets Mentioned

BETA
AF121265

Methods Mentioned

BETA
ubiquitination
PCR
protein assay
confocal microscopy
nuclear translocation
transfection

Software Mentioned

SPSS
Scion Image
Blast

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.