Adenohypophyseal and hypothalamic GABA B receptor subunits are downregulated by estradiol in adult female rats

Life Sciences
Estela B Rey-RoldánCarlos Libertun

Abstract

Gamma-aminobutyric acid (GABA) participates in neuroendocrine regulation. Since steroid hormones have been shown to modulate the GABAergic system, here we evaluated the effect of chronic in vivo estradiol administration on GABA B receptor (GABA(B)R) expression. GABA(B1) and GABA(B2) subunits were analyzed by Western Blot and RT-PCR, in hypothalami and anterior pituitaries of adult female rats: a) treated for 1 week with estradiol-valerate (a single dose of 100 mug /kg: E1), b) implanted with a 10 mg pellet of estradiol-benzoate for 5 weeks (E5) or c) on proestrous (P), d) ovariectomized (OVX). Pituitary GABA(B)R levels were correlated to a biological effect: baclofen, a GABA(B)R agonist, action on intracellular calcium titers ([Ca(2+)](i)) in pituitary cells. E5 pituitaries showed a significant decrease in the expression of GABA(B1) and GABA(B2) mRNAs compared to P. The GABA(B1a) splice variant of GABA(B1) was always more abundant than GABA(B1b) in this tissue. Similar to the pituitary, hypothalamic GABA(B1) and GABA(B2) mRNAs decreased in E5; this was confirmed at the protein level. In the hypothalamus GABA(B1b) was the main variant expressed in P rats, and was the one significantly sensitive to estradiol-induced decrease, as ...Continue Reading

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Citations

Feb 12, 2008·Cellular and Molecular Neurobiology·Victoria A Lux-LantosCarlos Libertun
Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
Feb 1, 2014·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Alfhild GrönbladhMathias Hallberg
Aug 19, 2008·Physiology & Behavior·Zhiping YuRebecca L Corwin
Oct 1, 2016·Molecular Metabolism·Ammy M SantiagoVanessa H Routh
Nov 1, 2007·American Journal of Physiology. Endocrinology and Metabolism·M M BonaventuraV A Lux-Lantos

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