PMID: 6172446Dec 1, 1981Paper

Adenosine 3',5'-monophosphate: a modulator of embryonic chick cartilage growth

The Journal of Clinical Investigation
W M Burch, H E Lebovitz

Abstract

We tested the hypothesis that cyclic AMP plays a significant role in modulating the growth of embryonic chick cartilage by determining whether cyclic AMP levels change in growing embryonic cartilage and whether cyclic AMP could stimulate embryonic cartilage growth in a long term in vitro organ culture. Cyclic AMP levels were low (0.1 pmol/mg wet wt) in 8-d chick embryo pelvic cartilage, and increased progressively through the 11th d of embryonic development at which time they reached a maximum (1.8 pmol/mg wet weight) and thereafter remained constant. We developed an in vitro organ culture system to determine whether cyclic AMP, a factor known to stimulate radiolabeled precursor incorporation into macromolecules in short-term studies does, in fact, stimulate growth of cartilage. Individual pelvic cartilages were isolated from 9-d chick embryos, placed in serum-free medium (BGJb-FJ modification) and incubated for 3 to 5 d during which time they increased in size (39 and 60% in length, respectively), wet weight (90 and 141%, respectively), and content of total soluble protein (30 and 48%, respectively). N6-monobutyryl cyclic AMP (BtcAMP) added to the medium caused a dose-dependent (0.05 to 1.0 mM) stimulation of growth. After 3 d...Continue Reading

References

Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T K Audhya, K D Gibson
Apr 2, 1976·Biochimica Et Biophysica Acta·M K DreznerH E Lebovitz
Feb 1, 1979·Endocrinology·M K Drezner, H E Lebovitz
Nov 1, 1979·Molecular and Cellular Biochemistry·J F WhitfieldB K Tsang
Jan 1, 1979·Differentiation; Research in Biological Diversity·M SolurshR M Pratt
May 1, 1978·The Journal of Clinical Endocrinology and Metabolism·M N FeinglosH E Lebovitz
Oct 9, 1970·Biochemical and Biophysical Research Communications·B D Wilson, R L Wright
Feb 21, 1980·The New England Journal of Medicine·L S Phillips, R Vassilopoulou-Sellin

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Citations

Jul 1, 1983·Calcified Tissue International·W M Burch, H E Lebovitz
Mar 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y IshikawaR E Wuthier
May 1, 1991·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·T Kantomaa, B K Hall
Feb 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·W M BurchM K Drezner
Feb 1, 1985·Metabolism: Clinical and Experimental·W M BurchR E Wuthier
Feb 1, 1984·The American Journal of Anatomy·T A Ballard, D M Biddulph

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