Adenosine-5'-triphosphate suppresses proliferation and migration capacity of human endometrial stem cells.

Journal of Cellular and Molecular Medicine
Svetlana SemenovaYuri A Negulyaev

Abstract

Extracellular ATP through the activation of the P2X and P2Y purinergic receptors affects the migration, proliferation and differentiation of many types of cells, including stem cells. High plasticity, low immunogenicity and immunomodulation ability of mesenchymal stem cells derived from human endometrium (eMSCs) allow them to be considered a prominent tool for regenerative medicine. Here, we examined the role of ATP in the proliferation and migration of human eMSCs. Using a wound healing assay, we showed that ATP-induced activation of purinergic receptors suppressed the migration ability of eMSCs. We found the expression of one of the ATP receptors, the P2X7 receptor in eMSCs. In spite of this, cell activation with specific P2X7 receptor agonist, BzATP did not significantly affect the cell migration. The allosteric P2X7 receptor inhibitor, AZ10606120 also did not prevent ATP-induced inhibition of cell migration, confirming that inhibition occurs without P2X7 receptor involvement. Flow cytometry analysis showed that high concentrations of ATP did not have a cytotoxic effect on eMSCs. At the same time, ATP induced the cell cycle arrest, suppressed the proliferative and migration capacity of eMSCs and therefore could affect the re...Continue Reading

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Citations

Jul 29, 2020·International Journal of Molecular Sciences·Marcin Szustak, Edyta Gendaszewska-Darmach
Oct 20, 2020·Frontiers in Cell and Developmental Biology·Mehdi NajarHassan Fahmi

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Methods Mentioned

BETA
PCR
electrophoresis
flow cytometry
FACS
Dot Plot
confocal microscopy

Software Mentioned

Axiovision
Cytexpert
Excel
GeneRunner

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