Adenosine A2A Receptors Mediate Anti-Inflammatory Effects of Electroacupuncture on Synovitis in Mice with Collagen-Induced Arthritis

Evidence-based Complementary and Alternative Medicine : ECAM
Qi-Hui LiJiang-Fan Chen

Abstract

To study the role of adenosine A2A receptor (A2AR) in mediating the anti-inflammatory effect of electroacupuncture (EA) on synovitis in collagen-induced arthritis (CIA), C57BL/6 mice were divided into five treatment groups: Sham-control, CIA-control, CIA-EA, CIA-SCH58261 (A2AR antagonist), and CIA-EA-SCH58261. All mice except those in the Sham-control group were immunized with collagen II for arthritis induction. EA treatment was administered using the stomach 36 and spleen 6 points, and stimulated with a continuous rectangular wave for 30 min daily. EA treatment and SCH58261 were administered daily from days 35 to 49 (n = 10). After treatment, X-ray radiography of joint bone morphology was established at day 60 and mouse blood was collected for ELISA determination of tumor necrosis factor alpha (TNF-α) levels. Mice were sacrificed and processed for histological examination of pathological changes of joint tissue, including hematoxylin-eosin staining and immunohistochemistry of A2AR expression. EA treatment resulted in significantly reduced pathological scores, TNF-α concentrations, and bone damage X-ray scores. Importantly, the anti-inflammatory and tissue-protective effect of EA treatment was reversed by coadministration of S...Continue Reading

References

Sep 30, 2000·Brain Research. Brain Research Reviews·A de MendonçaJ A Ribeiro
Aug 11, 2004·Best Practice & Research. Clinical Rheumatology·E Ernst
Mar 29, 2006·Rheumatology·L C HuberT Pap
Mar 8, 2007·Evidence-based Complementary and Alternative Medicine : ECAM·Yun-Kyoung YimJung-Eun Kim
Jan 22, 2008·Arthritis Research & Therapy·Kristin AndreasMichael Sittinger
Mar 5, 2010·The Journal of Allergy and Clinical Immunology·Amy JosephJohn Atkinson
Jun 1, 2010·Nature Neuroscience·Nanna GoldmanMaiken Nedergaard
Aug 27, 2010·Trends in Molecular Medicine·Elena NeumannUlf Müller-Ladner
Mar 24, 2011·Current Opinion in Rheumatology·Faye A H Cooles, John D Isaacs
Dec 6, 2011·The American Journal of Pathology·Aránzazu MedieroBruce N Cronstein
Nov 28, 2012·The Journal of Pain : Official Journal of the American Pain Society·Takahiro TakanoMaiken Nedergaard

❮ Previous
Next ❯

Citations

Mar 5, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Tian-Shen YeJian-Qiao Fang
Apr 9, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Zhong-Heng DuTian-Shen Ye
Jan 22, 2021·Pflügers Archiv : European journal of physiology·Maísa Maria Spagnol TrentoDaniel Fernandes Martins
Aug 28, 2021·International Journal of Molecular Sciences·Rebecca SohnZsuzsa Jenei-Lanzl

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
X-ray
ELISA
gene knockout

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.