PMID: 3997560Jan 1, 1985Paper

Adenosine deaminase activity associated with phagocytic vacuoles. Cytochemical demonstration by electron microscopy

Histochemistry
G TritschK L Bielat

Abstract

Adenosine deaminase activity has been localized within the cell membrane and it surrounds phagocytic vacuoles in mouse macrophages. Adenosine deaminase is thus strategically located to direct metabolic flux through the enzymes of the purine catabolic pathway. Xanthine oxidase, a key enzyme of this pathway, produces superoxide during its reaction with its substrates. Enzyme activity was visualized for electron microscopy by means of hydrolysis of 6-Chloropurine ribonucleoside to produce Cl-, which is precipitated with Ag+. The latter is converted into Ag0 by light, and the resulting deposit is visualized with the electron microscope.

References

Mar 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K Nakatsu
Jan 1, 1972·Pathologia et microbiologia·S J Mustafa
Jan 1, 1968·Archives of Biochemistry and Biophysics·H P BaerJ Gillis
Jan 1, 1980·Annual Review of Biochemistry·J A Badwey, M L Karnovsky
Mar 21, 1983·Life Sciences·G L Tritsch, P W Niswander
Oct 1, 1983·The Journal of Experimental Medicine·B N CronsteinR Hirschhorn
Mar 1, 1984·Clinical Immunology and Immunopathology·B N CronsteinR Hirschhorn
Jan 1, 1983·Annals of the New York Academy of Sciences·G L Tritsch, P W Niswander
Jul 1, 1980·The Journal of Experimental Medicine·R J Soberman, M L Karnovsky

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Citations

Apr 11, 1989·Molecular and Cellular Biochemistry·K Bielat, G L Tritsch
Aug 1, 1991·Immunology Letters·R Franco
Jul 2, 2003·Bioscience, Biotechnology, and Biochemistry·Kiyoshi OkuyamaToshitada Noguchi
Jan 1, 1985·Annals of the New York Academy of Sciences·G L Tritsch, P W Niswander

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