Adenosine deaminase and adenosine kinase expression in human glioma and their correlation with glioma‑associated epilepsy

Molecular Medicine Reports
Jun HuangXiaoyan Long

Abstract

The aim of the present study was to investigate adenosine deaminase (ADA) and adenosine kinase (ADK) expression in human glioma and to explore its correlation with glioma‑associated epilepsy. Tumor tissues (n=45) and peritumoral tissues (n=14) were obtained from glioma patients undergoing surgery. Normal control tissues (n=8) were obtained from brain trauma patients. The disease grade was determined by histological evaluation and the degree of tumor invasion was evaluated using immunofluorescence analyses. mRNA and protein expression of ADA and ADK were evaluated using reverse transcription quantitative polymerase chain reaction or western blot analysis, respectively. Based on histological evaluations, four cases were classified as Grade I gliomas, 18 cases as Grade II, 17 cases as Grade III and six cases were considered Grade IV. Increased ADA and ADK expression was observed in tumor tissues. ADA was predominantly distributed in the cytoplasm of tumor cells, whereas ADK was detected in the cytoplasm as well as in the nuclei. ADA and ADK levels were upregulated in patients with Grade II and Grade III gliomas compared to those in control subjects (p<0.05). In addition, tumor invasion was detected in peritumoral tissues. The numb...Continue Reading

References

Aug 1, 1994·British Journal of Cancer·V BardotC Luccioni
Jun 13, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·K LoteH Hirschberg
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·A HuberD Boison
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 12, 2002·Current Treatment Options in Oncology·V W Stieber
Feb 1, 2003·Lancet·Anthony BehinJean-Yves Delattre
Oct 19, 2004·Neuroscience Letters·Denise E FedeleDetlev Boison
Jul 3, 2007·Clinical Neurology and Neurosurgery·Laura M LynamJames A Wilkens
Jul 10, 2007·Acta Neuropathologica·David N LouisPaul Kleihues
Sep 20, 2007·European Journal of Pharmacology·Satoko OhkuboNorimichi Nakahata
Mar 14, 2009·The International Journal of Neuroscience·Sadrettin PenceUner Tan
Jul 30, 2009·Acta Oncologica·Shala Ghaderi BerntssonAnja Smits
Jul 31, 2012·Neurology·Tanya I YuenTerence J O'Brien
Jan 5, 2013·Pharmacology, Biochemistry, and Behavior·Anna Maria SiebelCarla Denise Bonan
Mar 1, 2013·Epilepsy Currents·Detlev Boison
Apr 18, 2013·Pharmacological Reviews·Detlev Boison
Apr 30, 2013·Handbook of Clinical Neurology·H A JinnahGeorges Van Den Berghe
Nov 26, 2013·Journal of Neurosurgery·Hai-Ying ShenDetlev Boison
Jan 1, 2014·Brain : a Journal of Neurology·Johan PalludGilles Huberfeld
Jun 7, 2014·Pharmacological Reports : PR·Mariusz J ŚwiąderJarogniew J Łuszczki

❮ Previous
Next ❯

Citations

Dec 22, 2019·Journal of Cellular and Molecular Medicine·Michelle ZallesRheal A Towner
Nov 18, 2018·International Journal of Molecular Sciences·Mercedes Garcia-GilMaria Grazia Tozzi
Sep 22, 2020·Journal of Caffeine and Adenosine Research·David N RuskinSusan A Masino
May 8, 2021·Neurochemistry International·Madhuvika MuruganDetlev Boison
Jun 1, 2021·Frontiers in Pharmacology·Mercedes Garcia-GilMaria Grazia Tozzi

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection
protein assay
light microscopy
deamination

Software Mentioned

Excel
SPSS

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Related Papers

Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia
Ligang JiangGuohua Hu
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Liu YangYilu Gao
© 2021 Meta ULC. All rights reserved