PMID: 7580858Jan 1, 1995Paper

Adenosine diphosphate glucose pyrophosphorylase genes in wheat: differential expression and gene mapping

Planta
C AinsworthM D Gale

Abstract

A full-length cDNA clone representing the large (shrunken-2) subunit of ADP-glucose pyrophosphorylase (AGP; EC 2.7.7.27) has been isolated from a cDNA library prepared from developing grain of hexaploid wheat (Triticum aestivum L., cv. Chinese Spring). The 2084-bp cDNA insert contains an open reading frame of 1566 nucleotides and primer-extension analysis indicated that the 5' end is 10 nucleotides shorter than the mRNA. The deduced protein contains 522 amino acids (57.8 kDa) and includes a putative transit peptide of 62 amino acids (6.5 kDa). The similarity of the deduced protein to the small subunit of AGP and to other AGP genes from plants and microorganisms is discussed. Northern hybridisation shows that the Agp1 genes (encoding the small subunit in the wheat endosperm) and the Agp2 genes (encoding the large subunit in the wheat endosperm) are differentially expressed in the wheat grain. Transcripts from both gene sets accumulate to high levels in the endosperm during grain development with the majority of the expression in the endopsperm rather than the embryo and pericarp layers. Although enzyme activity is detected in developing grains prior to 10 d post anthesis, only the Agp1 genes are active at this time (the Agp2 gen...Continue Reading

Citations

Jul 9, 2004·Genetics·Katherine S CaldwellWayne Powell
May 21, 2014·Journal of Genetics·Parviz FasahatWickneswari Ratnam
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·T W GreeneT W Okita
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·T W Greene, L C Hannah
Jan 1, 2003·Journal of Experimental Botany·M J EmesI J Tetlow
Jun 16, 2009·Journal of Plant Physiology·Paul TickleCaroline G Bowsher
Jun 15, 2016·Genome Génome / Conseil National De Recherches Canada·Xiao-Wei ZhangYou-Liang Zheng
Nov 27, 2004·Plant & Cell Physiology·Nicole Schupp, Paul Ziegler
Jul 15, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Gautam Saripalli, Pushpendra Kumar Gupta
Feb 15, 2018·Frontiers in Genetics·Jun GuoJianmin Song
Aug 6, 2020·Frontiers in Plant Science·Danisa M L FerreroAlberto A Iglesias

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