Adenosine diphosphate-sensitive P2Y11 receptor inhibits endothelial cell proliferation by induction of cell cycle arrest in the S phase and induces the expression of inflammatory mediators

Journal of Cellular Biochemistry
Xiaobin ChenZhilin Xiao

Abstract

Extracellular adenosine diphosphate (ADP) mediates a wide range of physiological effects as an extracellular signaling molecule, including platelet aggregation, vascular tone, cell proliferation, and apoptosis by interacting with plasma membrane P2 receptors. However, the effect of ADP on cell proliferation was contradictory. In this study, we found that ADP significantly inhibited cell proliferation of human umbilical vein endothelial cells at high concentrations (50 to 100 µM). Treatment with ADP did not induce cell apoptosis but instead induced cell cycle arrest in the S phase, which may be partly due to the downregulation of cyclin B1. The inhibition of cell proliferation was blocked by suramin, a nonspecific antagonist of the P2 receptors, and high concentrations of ADP significantly upregulated the messenger RNA (mRNA) and protein expression of P2Y11 in endothelial cells. Moreover, the downregulation of P2Y11 by RNA interference reversed the inhibition of cell proliferation. In addition, ADP (100 µM) can induce the formation of cytosolic autophagy in endothelial cells and a rapid phosphorylation of extracellular signal regulated kinase (ERK) 1/2, which is a canonical signal molecule downstream of P2Y receptors, accompanie...Continue Reading

References

Jan 1, 1992·Circulation Research·P Van DaeleJ M Boeynaems
Jul 6, 2000·British Journal of Pharmacology·Z Mallat, A Tedgui
Mar 9, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Geoffrey Burnstock
Nov 27, 2002·Journal of Cardiovascular Pharmacology·Lingwei WangDavid Erlinge
Feb 10, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sundararajah ThevanantherSaul J Karpen
Jan 18, 2006·The Journal of Investigative Dermatology·Kristina SeiffertRichard D Granstein
Aug 24, 2006·The Journal of Biological Chemistry·Iliana MoreschiAntonio De Flora
Mar 28, 2008·Purinergic Signalling·David Erlinge, Geoffrey Burnstock
Feb 4, 2009·Circulation·Cleide Gonçalves da SilvaElzbieta Kaczmarek
Aug 22, 2009·Journal of Neurochemistry·Ariadna G BattistaMaría Paula Faillace
Jul 28, 2010·Oncogene·J Stagg, M J Smyth
Sep 3, 2014·Journal of Neuroinflammation·Clara QuintasGlória Queiroz
Dec 3, 2014·Biochimica Et Biophysica Acta·Sébastien RogerLin-Hua Jiang
Apr 4, 2015·Molecular Pharmacology·Kenneth A JacobsonEvgeny Kiselev
Nov 1, 2015·Neuropharmacology·Ivar von Kügelgen, Kristina Hoffmann
Jun 2, 2016·Purinergic Signalling·Karin Dreisig, Birgitte Rahbek Kornum
Oct 21, 2016·Clinical and Experimental Pharmacology & Physiology·Fan Jiang
Feb 19, 2017·Molecular and Cellular Biochemistry·Maria D DănilăAdrian Sturza
Aug 5, 2017·Journal of Cellular Physiology·Antonella MuscellaSanto Marsigliante
Oct 8, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Huanhuan ZhouXiaojia Wang

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