PMID: 9435605Jan 22, 1998Paper

Adenosine-induced hyperpolarization in guinea pig coronary artery involves A2b receptors and KATP channels

The American Journal of Physiology
V N Mutafova-Yambolieva, K D Keef

Abstract

The role of P1 purinoceptor subtypes, the adenylyl cyclase (AC) pathway, and ATP-sensitive K+ (KATP) channels in adenosine (Ado)-induced membrane hyperpolarization was investigated in isolated segments of the guinea pig coronary artery using conventional microelectrode techniques. Ado (1-100 microM) elicited concentration-dependent hyperpolarization (half-maximal effective concentration 7.5 +/- 0.5 microM) that averaged 28.6 +/- 2.9 mV at 100 microM Ado. The A1 selective agonist N6-cyclopentyladenosine (CPA), the A1/A2 agonist 2-chloroadenosine, and the A2a/A2b agonist 5-(N-ethylcarboxamido)adenosine (NECA) each induced glibenclamide (3 microM)-sensitive hyperpolarization at 10 microM. However, the selective A2a-receptor agonists CGS-21680 and N6-[2-(3,5-dimethoxyphenyl)-2-(2-methoxyphenyl]ethyladenosine (10 microM each) were without effect. Responses to CPA and NECA were significantly reduced by the AC inhibitor SQ-22,536 (100 microM). Activation of the AC-adenosine 3',5'-cyclic monophosphate (cAMP)-protein kinase A (PKA) pathway by four additional methods, i.e., 1) forskolin (0.3-1 microM), 2) isoproterenol (0.1-1 microM), 3) combined milrinone (0.4 microM) and rolipram (30 microM), and 4) combined N6-phenyladenosine 3',5'-mo...Continue Reading

References

May 1, 1992·British Journal of Pharmacology·D M EckmanK D Keef
Jul 1, 1990·Physiological Reviews·R A Olsson, J D Pearson
Sep 10, 1995·Archives of Biochemistry and Biophysics·V C ManganielloE Degerman
Feb 1, 1995·Journal of Cardiovascular Pharmacology·H A OlanrewajuS J Mustafa
Oct 1, 1993·Trends in Pharmacological Sciences·M G Collis, S M Hourani
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·T Kleppisch, M T Nelson
Feb 1, 1963·The American Journal of Physiology·R M BERNE

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Citations

Oct 25, 2000·American Journal of Physiology. Heart and Circulatory Physiology·C W ChenT R Hsiue
Jun 18, 1999·British Journal of Pharmacology·B K Kemp, T M Cocks
Feb 22, 2001·American Journal of Physiology. Renal Physiology·A NishiyamaL G Navar
Sep 29, 2012·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Dovenia S PonnothS Jamal Mustafa
Aug 14, 2021·Pflügers Archiv : European journal of physiology·Simon Schemke, Cor de Wit

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