PMID: 7529114Sep 1, 1994Paper

Adenosine-induced hyperpolarization of the membrane voltage in rat mesangial cells in primary culture

British Journal of Pharmacology
H PavenstädtP Schollmeyer

Abstract

1. The effect of adenosine on membrane voltage and ion currents was studied in rat mesangial cells in primary culture. Membrane voltage was measured with the patch clamp technique in the slow- or fast whole cell configuration. The resting membrane voltage of mesangial cells was -48 +/- 0.5 mV. Adenosine (10(-8)-10(-3) M) induced a sustained and concentration-dependent hyperpolarization of membrane voltage (ED50 approximately 6 x 10(-7) M). Adenosine (10(-5) M) hyperpolarized the membrane voltage by 14 +/- 0.5 mV. During the hyperpolarization ion currents were monitored simultaneously. An increase of the outward current by 51 +/- 11% was observed. 2. An increase of the extracellular K+ concentration (from 3.6 to 18.6 M) caused a depolarization of membrane voltage to -34 +/- 2 mV. In the presence of increased K+ the hyperpolarization of membrane voltage induced by adenosine was significantly attenuated by 61 +/- 5%. The K(+)-channel blocker, Ba2+ (5 x 10(-3) M) depolarized membrane voltage to -24 +/- 2 mV. In the presence of Ba2+ the adenosine-induced hyperpolarization was significantly inhibited by 72 +/- 8%. 3. Preincubation of the adenosine antagonist, 8-phenyltheophylline (10(-4) M) significantly inhibited the adenosine (10(-...Continue Reading

References

Apr 1, 1975·The Journal of General Physiology·A Marty, A Finkelstein
Jan 1, 1992·Journal of Cardiovascular Pharmacology·M Seiss-GeuderJ Daut
Jan 1, 1989·Journal of Cellular Physiology·S G KremerK L Skorecki
Jan 1, 1989·Renal Physiology and Biochemistry·J M Pfeilschifter
Oct 1, 1989·Physiological Reviews·P MenéM J Dunn
Jun 1, 1989·Kidney International·A OliveraJ M López-Novoa
Oct 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D Schlondorff
Apr 1, 1987·Naunyn-Schmiedeberg's Archives of Pharmacology·M FreissmuthW Schütz
Jun 19, 1987·European Journal of Pharmacology·J M PalaciosA Probst
Dec 1, 1986·The Journal of Clinical Investigation·T OkudaK Kurokawa
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C LondosJ Wolff
Mar 1, 1983·Kidney International·J I Kreisberg, M J Karnovsky

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Citations

Feb 1, 1996·Pflügers Archiv : European journal of physiology·R CermakE Schlatter
Nov 23, 2013·Purinergic Signalling·Geoffrey BurnstockMatthew A Bailey
Dec 13, 2005·Neurochemical Research·Dietrich van Calker, Knut Biber
Oct 2, 2002·Acta Physiologica Scandinavica·A E G PerssonA Ollerstam
Jul 23, 1998·Physiological Reviews·J D Stockand, S C Sansom

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