PMID: 9654340Jul 8, 1998Paper

Adenosine induces apoptosis by inhibiting mRNA and protein synthesis in chick embryonic sympathetic neurons

Neuroscience Letters
J S KulkarniA R Wakade

Abstract

Our previous work has established that adenosine is toxic to chick embryonic sympathetic neurons and kills freshly plated neurons by a process of apoptosis. Although the exact mechanism remains unknown, we found that phosphorylation of adenosine was essential to the toxicity. Using markers for RNA ([3H]uridine) and protein ([35S]methionine) synthesis we demonstrate here that in freshly plated sympathetic neurons adenosine inhibits RNA and protein synthesis by about 50%. The inhibitory effects of adenosine on RNA and protein synthesis, and increased ATP synthesis were blocked by adenosine kinase inhibitor, suggesting that phosphorylated products are responsible for inhibition of RNA and protein synthesis and cell death. Adenosine-induced inhibition of RNA and protein synthesis in neuronal cells provides a new role for adenosine in the regulation of cell function.

References

Mar 1, 1977·Biochemical Pharmacology·R P AgarwalR E Parks
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R HirschhornA Ellenbogen
Feb 1, 1973·Analytical Biochemistry·G R Schacterle, R L Pollack

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Citations

Nov 9, 2000·Journal of Neuroscience Research·T Imura, S Shimohama
Feb 8, 2007·Pflügers Archiv : European journal of physiology·Olivier M NiemoellerFlorian Lang
Mar 31, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michael FountoulakisGert Lubec
Sep 30, 2000·Brain Research. Brain Research Reviews·A de MendonçaJ A Ribeiro
Mar 28, 1997·Molecular and Cellular Endocrinology·M D LewisM F Scanlon
May 4, 2012·Applied Immunohistochemistry & Molecular Morphology : AIMM·Anna SupernatAnna Zaczek
Jan 12, 2011·Cellular and Molecular Life Sciences : CMLS·Geoffrey Burnstock, Henning Ulrich
Mar 13, 2012·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Yoon-Ha KimChan-Wha Kim
May 9, 2015·Autonomic Neuroscience : Basic & Clinical·Cristina Giaroni
May 21, 2015·Purinergic Signalling·Geoffrey Burnstock, Nicholas Dale
Apr 3, 2001·Expert Opinion on Investigational Drugs·D K von Lubitz
Sep 23, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·E O HarringtonS Rounds

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis