Adenosine inhibits DNA synthesis stimulated with TSH, insulin, and phorbol 12-myristate 13-acetate in rat thyroid FRTL-5 cells

Journal of Cellular Physiology
M VainioK Törnquist

Abstract

Adenosine has been shown to modulate cell proliferation in FRTL-5 thyroid cells, although the mechanisms by which this interaction occurs is still unclear. In the present study we investigated the effects of adenosine on the 3H-thymidine incorporation, cell cycle kinetics, and expression of the transcription factor c-Fos in cells stimulated via three different mitogenic pathways, i.e., by thyroid stimulating hormone (TSH) [adenosine 3',5'-cyclic monophosphate(cAMP)], insulin (tyrosine kinase), or phorbol 12-myristate 13-acetate (protein kinase C). Addition of adenosine to cells grown in medium containing hormones and serum did not inhibit the incorporation of 3H-thymidine. If adenosine was added to hormone-deprived cells together with any of the tested mitogens, the stimulation of the 3H-thymidine incorporation was inhibited in a dose-dependent manner. The inhibition was significantly lower when the cells were preincubated with TSH or insulin for 48 h. Flow cytometric studies showed that adenosine evoked an inhibition of the cells in the G0/G1 phase. Submaximal doses of adenosine (10 nM-10 microM) were able to induce c-Fos expression in FRTL-5 cells. However, the mitogen-induced expression of c-Fos was not reduced by maximal do...Continue Reading

References

Dec 1, 1991·Baillière's Clinical Endocrinology and Metabolism·J E DumontP P Roger
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·F S Ambesi-ImpiombatoH G Coon
Aug 24, 1995·Biochemical and Biophysical Research Communications·M P AbbracchioF Cattabeni
Feb 1, 1996·Journal of Cellular Physiology·K TörnquistB Dugué
Mar 1, 1996·Hypertension·R K DubeyE K Jackson

❮ Previous
Next ❯

Citations

Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
Aug 26, 1998·Thyroid : Official Journal of the American Thyroid Association·V TasevskiA Simpson
Jan 17, 2014·Cardiovascular Drugs and Therapy·Ryszard T SmolenskiPaul J R Barton
Dec 20, 2002·Endocrine Research·Małgorzata KarbownikKrystyna Fabianowska-Majewska
Apr 2, 2008·Biological & Pharmaceutical Bulletin·Byung-Cheol LeeSe-Young Ahn

❮ Previous
Next ❯

Related Concepts

Related Feeds