Adenosine is inherently favored as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing

Biochemistry
Elena ZelinScott K Silverman

Abstract

We previously used in vitro selection to identify the 7S11 deoxyribozyme, which catalyzes formation of 2',5'-branched RNA using a branch-site adenosine nucleophile and a 5'-triphosphate electrophile. An unanswered question is whether the use of branch-site adenosine is inherently preferred or a chance event during the particular selection experiment. Here we have found that deoxyribozymes newly selected to use uridine as the branch-site RNA nucleotide in a structural context that resembles natural RNA splicing instead prefer a branch-site adenosine, although adenosine was never available during the selection itself. Our results support a chemical basis for nature's choice of the branch-site nucleotide, which is almost always adenosine in group II introns and the spliceosome.

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Citations

Jul 4, 2009·Accounts of Chemical Research·Scott K Silverman
Sep 24, 2008·Organic Letters·Timothy P Mui, Scott K Silverman
Jul 26, 2008·Chemical Communications : Chem Comm·Scott K Silverman
Mar 18, 2010·Chemical Communications : Chem Comm·Amit Sachdeva, Scott K Silverman
Jan 25, 2011·Chemical Communications : Chem Comm·Kennedy O Alila, Dana A Baum
Aug 27, 2010·Nucleic Acids Research·Christine S LeeScott K Silverman
Jul 11, 2008·Expert Opinion on Biological Therapy·Weihua Pan, Gary A Clawson
Dec 26, 2006·Blood Cells, Molecules & Diseases·Henrik Nielsen, Steinar D Johansen
Sep 19, 2007·Chembiochem : a European Journal of Chemical Biology·Elena Zelin, Scott K Silverman
Jan 25, 2008·Angewandte Chemie·P I PradeepkumarScott K Silverman
Jul 25, 2007·Biopolymers·Claudia Höbartner, Scott K Silverman
Aug 19, 2007·Angewandte Chemie·Claudia Höbartner, Scott K Silverman
Mar 31, 2007·Angewandte Chemie·Dana A Baum, Scott K Silverman
Oct 12, 2017·Science in Context·Cynthia Brokaw
Aug 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carolin P M ScheitlClaudia Höbartner
Oct 7, 2017·Chemical Communications : Chem Comm·Tucker J CarrocciAaron A Hoskins
Sep 19, 2020·Chemical Society Reviews·Ronald Micura, Claudia Höbartner
May 16, 2014·Journal of the American Chemical Society·Lea BüttnerClaudia Höbartner

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