Adenosine modulates cell proliferation in human colonic carcinoma. II. Differential behavior of HT29, DLD-1, Caco-2 and SW403 cell lines

European Journal of Pharmacology
V LelièvreJ Falcòn

Abstract

In a previous study, we provided evidence that extracellular adenosine modulates growth of the poorly differentiated colonic adenocarcinoma cells HT29 and proposed that adenosine A1 receptors might mediate proliferative effects. We now extend our investigations to a group of colonic adenocarcinoma cells at different stages of enterocytic differentiation. In HT29, DLD-1, Caco-2 and SW403, proliferation was decreased in the presence of adenosine deaminase (5 or 10 U/ml), 5'-N-ethylcarboxamido-adenosine (NECA; 1 microM), xanthine amine congener and 8-phenyltheophylline (both at 1 nM or 1 microM). NECA stimulated cAMP accumulation in all cell lines except for HT29. In the presence of forskolin (adenyl cyclase activator) cAMP accumulation was inhibited at sub-nanomolar concentrations of NECA and stimulated at micromolar concentrations in all four cell lines. The inhibitory response disappeared in the presence of 50 nM cyclopentyladenosine (CPA). The binding of [3H]cyclopentyl-1,3-dipropylxanthine and [3H]NECA was also investigated in the four cell lines. Results of displacement experiments were consistent with the idea that poorly differentiated cells with high proliferation rates (e.g. HT29) express mainly adenosine A receptors. In...Continue Reading

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Citations

Mar 29, 2001·BioFactors·R A MittalH E Khoo
Aug 3, 2007·Journal of Cancer Research and Clinical Oncology·Li WangPing Zhou
Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
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Jul 24, 2007·Biochimica Et Biophysica Acta·Elizandra BraganholAna M O Battastini
Sep 21, 2007·Cancer Investigation·Denisa StreitováVladimír Znojil
Sep 23, 2014·Mediators of Inflammation·Fariborz BahramiJean Sévigny
Mar 10, 2007·Journal of Cellular Physiology·Stefania GessiPier Andrea Borea
Feb 5, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jozef SpychalaBeverly S Mitchell
Jul 2, 2003·Cellular Signalling·Gunnar Schulte, Bertil B Fredholm

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