Adenosine modulates N-methyl-D-aspartate-stimulated hippocampal nitric oxide production in vivo

Stroke; a Journal of Cerebral Circulation
A BhardwajR J Traystman

Abstract

Adenosine acts presynaptically to inhibit release of excitatory amino acids (EAAs) and is thus considered to be neuroprotective. Because EAA-stimulated synthesis of nitric oxide (NO) may play an important role in long-term potentiation and excitotoxic-mediated injury, we tested the hypotheses that adenosine agonists attenuate basal and EAA-induced NO production in the hippocampus in vivo and that adenosine A1 receptors mediate this response. Microdialysis probes were placed bilaterally into the CA3 region of the hippocampus of adult Sprague-Dawley rats under pentobarbital anesthesia. Probes were perfused for 5 hours with artificial cerebrospinal fluid containing 3 mumol/L [14C]L-arginine. Recovery of [14C]L-citrulline in the effluent was used as a marker of NO production. In 10 groups of rats, time-dependent increases in [14C]L-citrulline recovery were compared between right- and left-sided probes perfused with various combinations of N-methyl-D-aspartate (NMDA), adenosine agonists, adenosine antagonists, and the NO synthase inhibitor N omega-nitro-L-arginine methyl ester (L-NAME). Recovery of [14C]L-citrulline during perfusion with artificial cerebrospinal fluid progressively increased to 141 +/- 27 fmol/min (+/- SEM) over 5 h...Continue Reading

References

Jul 1, 1992·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·V M SciottiD G Van Wylen
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F J NorthingtonR M Berne
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J A GallyG M Edelman
Oct 17, 1989·European Journal of Pharmacology·J GarthwaiteS Moncada
Aug 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D G Van WylenR M Berne
Apr 1, 1988·Trends in Pharmacological Sciences·B B Fredholm, T V Dunwiddie
Jan 1, 1984·Journal of Neurochemistry·F Fonnum
Apr 1, 1983·Brain Research Bulletin·R D Johnson, J B Justice
Mar 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·C IadecolaN A Lassen
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·J L DinermanS H Snyder
Sep 30, 1994·Science·A BensimonD Bensimon
May 1, 1993·The Journal of Comparative Neurology·J G ValtschanoffH H Schmidt
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C A SalvatoreR G Johnson
Jan 1, 1994·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J G ZhouH R Winn
May 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T MalinskiM Chopp

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Citations

Feb 13, 1999·European Journal of Pharmacology·D K Von Lubitz
Jun 4, 1999·European Journal of Pharmacology·D K von Lubitz
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·H H LamM V Sofroniew
Oct 22, 2005·Immunopharmacology and Immunotoxicology·Byung-Soo KooCheorl-Ho Kim
Mar 3, 2007·European Journal of Pharmacology·Lisa GodfreySusanna M O Hourani
Jan 31, 2006·Brain Research·Peter J MarroMaria Delivoria-Papadopoulos
Aug 24, 2005·Progress in Neurobiology·F X GuixF J Muñoz
Feb 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A BhardwajR C Koehler
Sep 29, 2000·American Journal of Physiology. Heart and Circulatory Physiology·A BhardwajR C Koehler

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