PMID: 8592203Nov 1, 1995Paper

Adenosine pre- and postsynaptic modulation of glutamate-dependent calcium activity in hypothalamic neurons

Journal of Neurophysiology
K ObrietanA N van den Pol

Abstract

1. Within the hypothalamus, adenosine has been reported to influence temperature regulation, sleep homeostasis, and endocrine secretions. The effects of adenosine on hypothalamic neurons have not been studied at the cellular level. Adenosine (5 nM-30 microM) showed no influence on intracellular Ca2+ or electrical activity in the presence of glutamate receptor antagonists D-2-amino-5-phosphonovalerate and 6-cyano-7-nitroquinoxaline-2,3-dione; consequently, we examined the role of adenosine in modulating the activity of glutamate in cultured hypothalamic neurons (n > 1,700) with fura-2 Ca2+ digital imaging and whole cell patch-clamp electrophysiology in the absence of glutamate receptor block. 2. When glutamate receptors were not blocked, adenosine (1-30 microM) and the selective adenosine A1 receptor agonist N6-cyclopentyl adenosine (CPA; 5 nM-1 microM) caused a large reduction in intracellular Ca2+ and electrical activity, suggesting that glutamate neurotransmission was critical for an effect of adenosine to be detected. Neuronal Ca2+ levels were reversibly depressed by CPA (50 nM), with a maximum depression of 90%, and these effects were blocked by coadministration of the A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthi...Continue Reading

Citations

Sep 28, 1998·The European Journal of Neuroscience·Z Y GaoB A Wolf
Apr 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jocelyn StockwellFrancisco S Cayabyab
Oct 27, 1998·Chronobiology International·J D Miller
Sep 10, 2014·The European Journal of Neuroscience·Hester C van DiepenTom Deboer
Aug 20, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Melvi M MethipparaDennis McGinty
Nov 22, 2000·Brain Research. Brain Research Reviews·A Ames

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