Abstract
To evaluate the effects of adenosine and related substances on events that occur during vasculogenesis and angiogenesis, using in vitro assays. Adenosine (ADO), inosine (INO, an adenosine catabolite), and 5'-(N-ethylcarboxamido) adenosine (NECA, an adenosine agonist) were evaluated for their effect on the proliferation of canine retinal microvascular endothelial cells (DRME), using a cell count assay. Also, these substances and ADO receptor selective agonists and antagonists were evaluated in an assay for DRME chemokinesis by measuring random migration into a wound made in a confluent cellular monolayer. Finally, the effects of these substances on DRME cord formation were evaluated in a 3-dimensional collagen gel. Bovine retinal extract (RE) was used as a positive control for all assays. There was no effect on proliferation of DRME by any of the substances related to adenosine, but VEGF yielded a 30% stimulation of proliferation. Retinal extract, 10 microM ADO and 1.2 nM VEGF stimulation of DRME migration was 2- to 2.5-fold greater than 10 microM INO yielded. In addition, a combination of 1.2 nM VEGF with 10 microM ADO exceeded the stimulation in migration by ADO only and VEGF only. The total length of tubes formed in the prese...Continue Reading
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