PMID: 3355858Apr 25, 1988Paper

Adenosine transporters in chromaffin cells: subcellular distribution and characterization

Biochimica Et Biophysica Acta
M TorresM T Miras-Portugal

Abstract

Adenosine transporters in freshly isolated and cultured chromaffin cells were quantified by the [3H]dipyridamole binding technique, showing a maximal bound capacity of 0.4 +/- 0.05 pmol/10(6) cells (240,000 +/- 20,000 transporters by cell). Scatchard analysis showed a similar affinity for [3H]dipyridamole in isolated cells and subcellular fractions (Kd = 5 +/- 0.6 nM). For enriched plasma membrane preparations and chromaffin granule membranes, the maximal binding capacities were also very similar, 2.3 +/- 0.3 and 1.8 +/- 0.4 pmol/mg protein, respectively. When [3H]nitrobenzylthioinosine was employed as a radioligand, the maximal bound capacity in cultured chromaffin cells was 0.053 +/- 0.004 pmol/10(6) cells (32,000 +/- 3000 transporters per cell) with a high affinity constant (Kd = 0.25 +/- 0.03 nM); similar values were obtained in all subcellular fractions (Kd = 0.1 +/- 0.01). Also, plasma and chromaffin granule membranes showed similar maximal binding values (0.4 +/- 0.06 pmol/mg protein). Photoincorporation studies with [3H]nitrobenzylthioinosine into plasma membrane polypeptides showed the presence of three molecular species of 115 +/- 10; 58 +/- 6 and 42 +/- 5 kDa. Chromaffin granule membranes showed only the 105 +/- 9 an...Continue Reading

References

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Citations

Oct 1, 1993·Cellular and Molecular Neurobiology·M D Fideu, M T Miras-Portugal
Sep 11, 2001·Journal of Biomedical Science·C J TsengC R Jan
Jan 15, 1991·Biochemical Pharmacology·W B MahonyT P Zimmerman
May 2, 1991·European Journal of Pharmacology·R A Cunha, A M Sebastião
Jan 1, 1993·Advances in Enzyme Regulation·J A BeltC R Crawford
Jan 1, 1990·Neurochemistry International·R P SenM T Miras-Portugal
May 4, 1990·Neuroscience Letters·M TorresM T Miras-Portugal
Jun 1, 1996·General Pharmacology·J A Thorn, S M Jarvis
Jun 1, 1990·Journal of Neurochemistry·E G DelicadoM T Miras-Portugal
Feb 1, 1993·Journal of Neurochemistry·R P SenM T Miras-Portugal
Jun 1, 1990·British Journal of Pharmacology·E CastroM P Gonzalez
Nov 1, 1992·Neurochemical Research·M D Fideu, M T Miras-Portugal
Dec 2, 2004·Nucleosides, Nucleotides & Nucleic Acids·R J TorresJ G Puig
Aug 15, 1992·Archives of Biochemistry and Biophysics·F Rodriguez-PascualM T Miras-Portugal

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