Adenosine triphosphate-dependent calcium uptake of synaptic vesicle fraction is largely due to contaminating microsomes

Journal of Biochemistry
T Tsudzuki


Ca2+ uptake by synaptic vesicle fractions isolated from bovine caudatolenticular nuclei and from rat brain was studied. The purified vesicle fractions from both materials took up very little Ca2+ even in the presence of ATP and Mg2+, but the crude fractions took up Ca2+ actively, showing the maximum uptake around pH 7.0. Since the crude fractions were contaminated by microsomes, which are known to accumulate Ca2+ actively (Yoshida, H., Kadota, K., & Fujisawa, H. (1966) Nature 212, 291--292; Otsuka, M., Ohtsuki, I., & Ebashi, S. (1965) J. Biochem. 58, 188-190), the active uptake of Ca2+ appeared to be largely, if not wholly, due to microsomal contamination.


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