Adenoviral delivery of low-density lipoprotein receptors to hyperlipidemic rabbits: receptor expression modulates high-density lipoproteins

Metabolism: Clinical and Experimental
D R BrownJ M Hoeg

Abstract

Plasma concentrations of low-density lipoproteins (LDLs) and high-density lipoproteins (HDLs) are inversely related in several dyslipoproteinemias. To elucidate the interactions between these lipoproteins, we used a recombinant adenovirus (hLDLR-rAdV) to express human LDL receptors (hLDLRs) in LDL receptor-deficient rabbits. hLDLR-rAdV administration resulted in hepatocyte expression and a reduction of total, intermediate-density lipoprotein (IDL), and LDL cholesterol. In addition, we found that hLDLR-rAdV treatment induced (1) increased very-low-density lipoprotein (VLDL) cholesterol, (2) increased VLDL, IDL and LDL triglycerides, (3) decreased alpha- and pre-beta-migrating apolipoprotein E (apo E) and decreased pre-beta-migrating apo A-I at 2 to 4 days posttreatment, and (4) increased total plasma apo A-I and pre-beta-migrating apo A-I beginning 8 to 10 days posttreatment. Virtually all plasma apo A-I was present on alpha- and pre-beta-HDL. Pre-beta-HDL particles with size and electrophoretic properties consistent with nascent HDL demonstrated the greatest relative apo A-I enrichment following hLDLR-rAdV treatment. In summary, enhanced expression of hepatocyte LDLRs by hLDLR-rAdV treatment markedly altered apo A-I-containing ...Continue Reading

References

Jan 1, 1977·Annual Review of Biochemistry·J L Goldstein, M S Brown
Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Aug 1, 1992·Nature Genetics·H A JaffeM Perricaudet
Apr 11, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·A P PattersonH B Brewer
Jul 11, 1992·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·J R SchaeferH B Brewer
Aug 1, 1990·European Heart Journal·O L Francone, C J Fielding
Nov 9, 1989·The New England Journal of Medicine·D J Gordon, B M Rifkind
Apr 3, 1989·Biochimica Et Biophysica Acta·M A ClayP J Barter
Jan 15, 1987·Biochemical and Biophysical Research Communications·R BarbarasG Ailhaud
Apr 15, 1986·The American Journal of Cardiology·J M HoegD L Sprecher
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Feb 1, 1987·Molecular and Cellular Biology·J R de WetS Subramani
May 1, 1985·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·W C BreckenridgeA Kuksis
Mar 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·I C HsuB F Trump
Oct 1, 1966·Circulation·J W GofmanR Tandy
Jul 1, 1984·The American Journal of Cardiology·D L SprecherH B Brewer
Jan 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·L M BujaM S Brown
May 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·R E PitasR W Mahley
Oct 1, 1951·The American Journal of Medicine·D P BARRH A EDER

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Citations

Jul 30, 2005·Expert Opinion on Biological Therapy·Uli C Broedl, Daniel J Rader
Dec 21, 2000·Current Atherosclerosis Reports·D J Rader, U J Tietge
Jul 15, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·J R SchaeferW März
Oct 13, 2005·American Journal of Physiology. Endocrinology and Metabolism·Nandita S RaikwarMark A Deeg

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