PMID: 9664218Jul 17, 1998Paper

Adenoviral-mediated transfection of the Lac-Z gene into rat dissociated embryonic central nervous system cells before and after seeding

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
C CotC Levallois

Abstract

The adenovirus carrying a reporter gene--the Lac Z gene--is known to infect central nervous system (CNS) cells in primary cell cultures. The percentage of infected neurons with respect to the total number of neurons was studied in primary dissociated cultures as a function of the day of inoculation and the age of three rat CNS cultures: spinal cord, mesencephalon and cortex. Two methods of viral inoculation were compared: the first inoculation was performed on the cultured cell at 2, 3 or 6 days in vitro (DIV) whereas the second inoculation was performed on the cell suspensions before seeding. All the infected CNS cells has the same aspect as the control cultures. In the spinal cord and the mesencephalic cultures, the glial cells were preferentially infected, especially when the cells were inoculated at 6 DIV. In the cortical cultures, there were more infected neurons than infected glial cells. The number of CNS cells was lower when inoculation was performed at 6 DIV as compared with 3 DIV. Very few infected GABA cells were found in the cultures. A high percentage of infected neuronal cells relative to the total number of neuronal cells was found when infection of the three types of cultures was performed on the dissociated emb...Continue Reading

References

Jul 1, 1993·Trends in Neurosciences·R L Neve
Jan 1, 1995·British Medical Bulletin·E J Kremer, M Perricaudet
Nov 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L C Doering
Oct 1, 1994·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C LevalloisJ M Kamenka
Nov 1, 1993·Archives of Neurology·S T Suhr, F H Gage
Oct 1, 1993·The European Journal of Neuroscience·C CaillaudY Berwald-Netter
Mar 1, 1993·Nature Genetics·S AkliM R Peschanski
Aug 1, 1996·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C LevalloisA Privat
Jan 1, 1995·Restorative Neurology and Neuroscience·F SmithX O Breakefield

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Citations

Dec 31, 1998·Current Opinion in Neurology·M Giménez y Ribotta, A Privat

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