Adenoviral transfer of human interleukin-10 gene in lethal pancreatitis

World Journal of Gastroenterology : WJG
Zi-Qian ChenSheng-Dao Zhang

Abstract

To evaluate the therapeutic effect of adenoviral-vector-delivered human interleukin-10 (hIL-10) gene on severe acute pancreatitis (SAP) rats. Healthy Sprague-Dawley (SD) rats were intraperitoneally injected with adenoviral IL-10 gene (AdvhIL-10), empty vector (Adv0) or PBS solution. Blood, liver, pancreas and lung were harvested on the second day to examine hIL-10 level by ELISA and serum amylase by enzymatic assay. A SAP model was induced by retrograde injection of sodium taurocholate through pancreatic duct. SAP rats were then administered with AdvhIL-10, Adv0 and PBS solution by a single intraperitoneal injection 20 min after SAP induction. In addition to serum amylase assay, levels of hIL-10 and tumor necrosis factor-alpha (TNF-alpha) were detected by RT-PCR, ELISA and histological study. The mortality rate was studied and analyzed by Kaplan-Meier and log rank analysis. The levels of hIL-10 in the pancreas, liver and lung of healthy rats increased significantly after AdvhIL-10 injection (1.42 ng/g in liver, 0.91 ng/g in pancreas); while there was no significant change of hIL-10 in the other two control groups. The concentration of hIL-10 was increased significantly in the SAP rats after AdvhIL-10 injection (1.68 ng/g in liv...Continue Reading

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Citations

Apr 4, 2014·The American Journal of the Medical Sciences·Feihu ZhangEnqiang Mao
Dec 22, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Qingfeng ChenZhigang Tian
Jun 23, 2006·Der Unfallchirurg·A OberholzerW Ertel
Dec 11, 2014·World Journal of Gastroenterology : WJG·Jing LiCheng-Wei Tang

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