PMID: 11605941Oct 19, 2001Paper

Adenoviral vector transfection into the pulmonary epithelium after cecal ligation and puncture in rats

Anesthesiology
Yoram G WeissClifford S Deutschman

Abstract

Adenoviral-targeted gene delivery to respiratory epithelium can augment production of specific proteins. Therefore, it may be valuable in treating the acute respiratory distress syndrome. The authors tested the hypothesis that adenoviral vector uptake after cecal ligation and double puncture in rats, an animal model of the acute respiratory distress syndrome, is higher than that observed in controls that did not undergo operation ("nonoperated") or those that underwent a sham operation ("sham-operated"). Adenoviruses expressing green fluorescent protein or Lac-Z were delivered into the lungs of anesthetized rats via tracheal catheter. Animals were killed 24 or 48 h later. Histopathology and green fluorescent protein expression were examined using light of fluorescence microscopy. Cellular localization of Lac-Z was determined with electron microscopy or semithin sectioning. Viral receptor density and localization were determined using imunoblotting and immunohistochemistry. After cecal ligation and double puncture, rats were hypoxic and tachypneic. Alveoli were segmentally consolidated, contained proteinaceous debris and neutrophils, and had thickened septa. Administration of adenoviruses to rats that were sham-operated or under...Continue Reading

References

Mar 1, 1990·Journal of Biochemical and Biophysical Methods·A A CalderónR Muñoz
Jun 1, 1993·Current Opinion in Genetics & Development·K F Kozarsky, J M Wilson
Feb 1, 1993·Journal of Bioenergetics and Biomembranes·T R Flotte
May 14, 1993·Science·R C Mulligan
May 1, 1996·The European Respiratory Journal·P PelosiL Gattinoni
Aug 1, 1997·American Journal of Respiratory and Critical Care Medicine·J MalloyJ Lewis
May 4, 2000·The New England Journal of Medicine·UNKNOWN Acute Respiratory Distress Syndrome NetworkArthur Wheeler
May 4, 2000·The New England Journal of Medicine·L B Ware, M A Matthay
May 5, 2000·Biochemical Pharmacology·J M Bergelson

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Citations

Jul 21, 2005·Journal of Anesthesia·Zohar BrombergYoram G Weiss
Apr 21, 2006·Critical Care Medicine·Glenn K LevineSusan S Margulies
May 4, 2005·Inflammatory Bowel Diseases·Phyllissa Schmiedlin-RenEllen M Zimmermann
May 8, 2007·Shock·Stéphanie RignaultFrançois Feihl
Nov 12, 2005·American Journal of Physiology. Cell Physiology·Kenneth J CavanaughSusan S Margulies
Sep 18, 2002·The Journal of Clinical Investigation·Yoram G WeissClifford S Deutschman
Oct 31, 2007·Respiratory Research·Luminita EidYoram G Weiss
Jun 28, 2011·Critical Care : the Official Journal of the Critical Care Forum·James DevaneyJohn G Laffey
Aug 21, 2010·Journal of Leukocyte Biology·Laurie E KilpatrickClifford S Deutschman
Feb 11, 2015·Shock·M Melanie LyonsClifford S Deutschman
Sep 9, 2006·Respiration; International Review of Thoracic Diseases·Do Jin KimSeung Kyoon Woo
Dec 20, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kristen D Singleton, Paul E Wischmeyer
Jan 20, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kristen D Singleton, Paul E Wischmeyer
May 23, 2003·Anesthesia and Analgesia·Noor M Gajraj

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