PMID: 9525640Apr 3, 1998Paper

Adenovirus E1B 55K represses p53 activation in vitro

Journal of Virology
M E Martin, Arnold J Berk

Abstract

Adenovirus E1B 55K protein cooperates with E1A gene products to induce cell transformation. E1B 55K mediates its effects by binding to and inhibiting the transcriptional activation and growth-suppression functions of the tumor suppressor p53. Previous studies in vivo have suggested that E1B 55K has an active role in repressing p53 transcriptional activation and that this repression function is directed to specific promoters through E1B 55K's interaction with DNA-bound p53. Flag-tagged E1B 55K (e55K) was expressed with the baculovirus expression system and immunopurified. Gel filtration, velocity sedimentation centrifugation, and glutaraldehyde cross-linking indicated that e55K is a dimer with a nonglobular conformation. e55K bound directly to purified p53, causing an approximately 10-fold increase in p53 affinity for tandem binding sites. Using in vitro transcription assays reconstituted with purified p53, e55K, and HeLa cell nuclear extracts, we found that e55K specifically repressed p53 activation. These results demonstrate that as postulated from earlier transient expression experiments, E1B 55K is a specific repressor of transcription from a promoter with bound p53. Since HeLa nuclear extracts contain little detectable hist...Continue Reading

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Citations

Sep 22, 2006·Viral Immunology·Mingzhou Chen, Denis Gerlier
Feb 20, 2015·Journal of Virology·Roberta L TurnerDavid A Ornelles
Jul 16, 2019·The EMBO Journal·Ladan GheiratmandLaurence Pelletier
Jul 13, 2000·Molecular and Cellular Biology·Y LiuD Liao
Aug 21, 2002·Journal of Virology·Josephine N HaradaArnold J Berk
May 30, 2009·International Journal of Radiation Biology·Ping-Kun ZhouJing An
Feb 13, 2009·Journal of Virology·Andrew N Blackford, Roger J A Grand
Jun 19, 2012·Clinical Epigenetics·Elzbieta PorebaAnna Gozdzicka-Jozefiak
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Jun 6, 2006·Laboratory Investigation; a Journal of Technical Methods and Pathology·Vladimir V TernovoiGene P Siegal
Apr 19, 2008·The Journal of General Virology·Bodil ØsterPer Höllsberg
Nov 13, 2015·Viruses·Pei-Hsin ChengHeshan Sam Zhou
Nov 27, 2019·FEBS Letters·Paloma HidalgoRamón A Gonzalez

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