Adenovirus Infection of Human Enteroids Reveals Interferon Sensitivity and Preferential Infection of Goblet Cells

Journal of Virology
Mayumi K Holly, Jason G Smith

Abstract

Human adenoviruses (HAdV) are significant human pathogens. Although only a subset of HAdV serotypes commonly cause gastroenteritis in humans, most HAdV species replicate in the gastrointestinal tract. Knowledge of the complex interaction between HAdVs and the human intestinal epithelium has been limited by the lack of a suitable cell culture system containing relevant cell types. Recently, this need has been met by the stable and prolonged cultivation of primary intestinal epithelial cells as enteroids. Human enteroids have been used to reveal novel and interesting aspects of rotavirus, norovirus, and enterovirus replication, prompting us to explore their suitability for HAdV culture. We found that both prototype strains and clinical isolates of enteric and nonenteric HAdVs productively replicate in human enteroids. HAdV-5p, a respiratory pathogen, and HAdV-41p, an enteric pathogen, are both sensitive to type I and III interferons in human enteroid monolayers but not A549 cells. Interestingly, HAdV-5p, but not HAdV-41p, preferentially infected goblet cells. And, HAdV-5p but not HAdV-41p was potently neutralized by the enteric human alpha-defensin HD5. These studies highlight new facets of HAdV biology that are uniquely revealed...Continue Reading

References

Feb 1, 1976·Infection and Immunity·A Takeuchi, K Hashimoto
Apr 1, 1977·American Journal of Epidemiology·J P FoxM K Cooney
Aug 1, 1988·Journal of Virological Methods·D J Witt, E B Bousquet
Sep 1, 1984·Journal of Clinical Microbiology·I UhnooM E Johansson
Apr 1, 1983·American Journal of Epidemiology·H SchmitzW Heinrich
Jan 1, 1983·Journal of Medical Virology·J C de JongR G Firtzlaff
Jan 1, 1995·Current Topics in Microbiology and Immunology·V MautnerA Bailey
Jan 1, 1993·Archives of Virology·C T Tiemessen, A H Kidd
Jan 1, 1993·Archives of Virology·C T TiemessenA H Kidd
Nov 4, 2000·Journal of Clinical Microbiology·W XuD D Erdman
Oct 21, 2003·Nature Medicine·Anuj GaggarAndré Lieber
Mar 22, 2005·Nature Cell Biology·Johan H van EsHans Clevers
Sep 15, 2005·Journal of Virology·Yuanming Zhang, Jeffrey M Bergelson
Jan 15, 2008·Cell Host & Microbe·Jason G Smith, Glen R Nemerow
Dec 2, 2009·Journal of Medical Virology·Mpho MagwalivhaMaureen B Taylor
Mar 23, 2011·Nature Reviews. Microbiology·Charles L Bevins, Nita H Salzman
Apr 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Johanna PottMathias W Hornef
Oct 17, 2013·Diabetes·Natalia PetersenEelco J P de Koning
Apr 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Megan H OrzalliDavid M Knipe
Dec 30, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·K KosulinT Lion
Mar 5, 2016·PLoS Pathogens·Anshu P GounderJason G Smith
Aug 27, 2016·Science·Khalil EttayebiMary K Estes
Jan 11, 2017·Proceedings of the National Academy of Sciences of the United States of America·Kapil SaxenaMary K Estes
Feb 1, 2017·Proceedings of the National Academy of Sciences of the United States of America·Coyne G DrummondCarolyn B Coyne
Apr 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Bin LiHarry B Greenberg

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Citations

Nov 30, 2019·FEBS Letters·Silvio Hemmi, Katherine R Spindler
Oct 8, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sarah E BluttMary K Estes
Jan 31, 2020·PLoS Pathogens·Abimbola O Kolawole, Christiane E Wobus
Jun 24, 2019·Journal of Medical Virology·Hivylla L Dos Santos FerreiraLidio Gonçalves Lima-Neto
Apr 28, 2018·Viruses·Mayumi K Holly, Jason G Smith
Aug 7, 2019·Current Medicinal Chemistry·David C Brice, Gill Diamond
Aug 19, 2020·Nature Materials·Masayuki Fujii, Toshiro Sato
Jul 22, 2019·Veterinary Research·Talita Pilar ResendeConnie Gebhart
Nov 25, 2020·PLoS Pathogens·Karina DiazJason G Smith
Jan 29, 2021·The FEBS Journal·Valerie Cortez, Stacey Schultz-Cherry
Nov 15, 2020·Viruses·Inés García-RodríguezKatja C Wolthers
Apr 16, 2018·Current Opinion in Virology·Sasirekha RamaniMary K Estes
Sep 11, 2020·Proceedings of the National Academy of Sciences of the United States of America·Shih-Ching LinMary K Estes
Mar 9, 2019·Microbiology Spectrum·Alyssa C FascianoRalph R Isberg
Jun 3, 2021·Viruses·Victoria R TengeMary K Estes
Jul 25, 2021·International Journal of Molecular Sciences·Joseph AzarWassim Abou-Kheir
Oct 16, 2019·ACS Infectious Diseases·Rajiv S JumaniUjjini H Manjunatha
Aug 15, 2021·Advanced Drug Delivery Reviews·Connor O'FarrellHannah Batchelor
Aug 28, 2021·Microorganisms·Nanci Santos-FerreiraJoana Rocha-Pereira
Sep 18, 2021·Science Immunology·Johannes M JungMadeleine J Bunders
Sep 19, 2021·Journal of Molecular Biology·Wenqiang FanGuo-Li Ming
Nov 19, 2021·Frontiers in Cellular and Infection Microbiology·Inés García-RodríguezKatja C Wolthers

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