PMID: 11340577May 8, 2001Paper

Adenovirus-mediated gene therapy for bladder cancer in an orthotopic model using a dominant negative H-ras mutant

International Journal of Cancer. Journal International Du Cancer
T WatanabeN Kuzumaki

Abstract

It has been suggested that abnormal Ras function is important in the carcinogenesis and progression of bladder cancer. Our aim was to investigate the efficacy of transurethral inoculation of an adenovirus expressing the dominant negative H-ras mutant N116Y against orthotopically implanted human bladder-cancer cells in nude mice. We used a replication-defective adenovirus vector containing the beta-galactosidase gene (AdCMV-LacZ) as a control and the N116Y gene (AdCMV-N116Y) as the therapeutic vector under the transcriptional control of the cytomegalovirus promoter. We initially investigated the in vitro growth-suppressive effects of AdCMV-N116Y on 2 human bladder-cancer cell lines, KU-7 and UMUC-2. Thereafter, we examined the inhibitory effects of AdCMV-N116Y on the 2 orthotopically implanted cell lines in nude mice. Intravesically created, orthotopic human bladder cancers were established in female KSN athymic nude mice with 1x 10(7) cancer cells. Then, 2, 3 and 4 days following implantation, 1 x 10(9) pfu of AdCMV-LacZ or AdCMV-N116Y were administered transurethrally. In vitro growth assays revealed significant growth suppression (>95%) with apoptosis of target cells treated with AdCMV-N116Y compared to AdCMV-LacZ. Transureth...Continue Reading

References

Feb 1, 1976·The Journal of Urology·M A Mufson, R B Belshe
Jun 1, 1995·Molecular Biotechnology·F L Graham, L Prevec
Feb 1, 1996·The Journal of Urology·P E WerthmanA Shaked
Dec 1, 1996·The Journal of Surgical Research·T ShichinoheN Kuzumaki
Jan 1, 1997·World Journal of Urology·D A Corral, C J Logothetis
May 1, 1997·Lancet·R R Weichselbaum, D Kufe
Oct 10, 1998·International Journal of Cancer. Journal International Du Cancer·N SenmaruN Kuzumaki
Feb 6, 1999·International Journal of Cancer. Journal International Du Cancer·K KitaA Watanabe

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Citations

May 1, 2002·Current Opinion in Oncology·Suzanne E PattonHaluk Ozen
Aug 27, 2002·Journal of Clinical Pathology·A Lopez-BeltranR Montironi
Jul 23, 2011·Genes & Cancer·Alberto Fernández-Medarde, Eugenio Santos
Dec 14, 2016·Histopathology·Antonio Lopez-BeltranLiang Cheng
Jan 9, 2004·International Journal of Cancer. Journal International Du Cancer·Qinzhong ChenTomohiko Koyanagi

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