Adenovirus-mediated gene transfer of soluble vascular cell adhesion molecule to porcine interposition vein grafts

Circulation
S J ChenD W Muller

Abstract

The efficacy of aorto-coronary vein grafting is limited by early graft thrombosis and accelerated graft atherosclerosis. Direct adenovirus-mediated transfer of genes encoding inhibitory proteins may prevent or slow progression of vein graft disease. Recombinant adenoviruses containing the cDNA for the marker gene lacZ (Ad.CMVlacZ) or soluble vascular cell adhesion molecule (sVCAM) (Ad.CB-sVCAM) were used to infect segments of porcine jugular vein or human saphenous vein. Ex vivo testing showed expression of the introduced genes after incubation with Ad.CMVlacZ or Ad.CBsVCAM for periods from 1 to 24 hours, with an increase in transfection efficiency with increasing incubation time. Porcine jugular veins were then interposed as vascular grafts in the carotid arteries of four juvenile farm pigs after ex vivo gene transfer by incubation for 90 to 120 minutes with Ad.CMVlacZ or Ad.CBsVCAM. sVCAM-transfected carotid vein grafts were placed on one side and lacZ transfected veins were placed contralaterally as controls. Three days later, the vein graft segments were resected. Expression of the lacZ gene was confirmed by X-Gal chromagen staining and visualization by light and transmission electron microscopy. Gene expression was apparen...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P LemarchandR G Crystal
Apr 1, 1991·Journal of the American College of Cardiology·G M FitzGibbonW J Keon
Jun 1, 1991·The American Journal of Cardiology·J AmanoF Numano
Apr 1, 1990·Immunological Reviews·T M Carlos, J M Harlan
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M WilsonR C Mulligan
Nov 1, 1989·The Annals of Thoracic Surgery·J E Molina
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J Logan, T Shenk
Jul 1, 1993·The Journal of Clinical Investigation·L A KirshenbaumM D Schneider
Jan 1, 1991·Methods in Molecular Biology·F L Graham, L Prevec

❮ Previous
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Citations

Jun 22, 2000·Journal of Cellular Biochemistry·R T FranceschiR B Rutherford
Jul 14, 1999·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·M Chikada, M Jones
Sep 1, 1995·Journal of the American College of Cardiology·L J Feldman, J M Isner
May 18, 2004·Pathologie-biologie·M MazighiL J Feldman
Sep 24, 1998·Transplantation Proceedings·K S BlackJ Ollerenshaw
Feb 16, 2000·Lancet·S Ylä-Herttuala, J F Martin
Feb 22, 2001·Veterinary Immunology and Immunopathology·D F Morrison, M P Murtaugh
Oct 4, 2002·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Jennifer M Sarjeant, Marlene Rabinovitch
Jun 22, 2000·Molecular Medicine Today·M J Mann
Sep 3, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Rowan FlynnDavid A Dichek
Jul 21, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Rowan FlynnDavid A Dichek
May 17, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Tuomas T Rissanen, Seppo Ylä-Herttuala
Mar 20, 2003·Journal of Cardiac Surgery·Stephen J White, Andrew C Newby
May 15, 1998·Human Gene Therapy·D B SchneiderR L Geary
Jul 1, 1999·The Journal of Cardiovascular Nursing·S L Meyerson, L B Schwartz
Jun 14, 2000·Transplantation·S GojoC LeGuern
Jan 1, 1997·Fundamental & Clinical Pharmacology·D Stephan, E G Nabel
Oct 1, 1994·British Heart Journal·D W Muller
Mar 1, 1996·Heart·K M ChannonS E George
Jun 1, 1995·The Journal of Clinical Investigation·L J FeldmanJ M Isner
Jan 22, 2004·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Michael J Mann
Jul 29, 2000·Biochemical and Biophysical Research Communications·M SuzukiM Ohno
Sep 12, 2000·Current Cardiology Reports·M J Mann
Jan 5, 2001·Current Cardiology Reports·D G Cable, H V Schaff
Feb 28, 2007·Journal of the American College of Surgeons·Nicholas D AndersenFrank W Logerfo
Oct 29, 2002·The Annals of Thoracic Surgery·Cynthia K Chiu-PinheiroHartzell V Schaff
Feb 15, 2001·The Journal of Thoracic and Cardiovascular Surgery·V J DzauD P Griese
Dec 11, 2002·Cardiology Clinics·Michael J Mann, Victor J Dzau
Jun 25, 2010·Journal of Vascular Surgery·Peng YuC Keith Ozaki
Feb 1, 1997·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·U Hedin, E Wahlberg
Nov 11, 2014·Human Gene Therapy·Liang DuDavid A Dichek
May 4, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Song WanAndrew H Baker
Aug 16, 2000·Vascular Medicine·A Ehsan, M J Mann

❮ Previous
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