PMID: 9427504Jan 14, 1998Paper

Adenovirus-mediated gene transfer to cultured nodose sensory neurons

Brain Research. Molecular Brain Research
S S MeyrellesM W Chapleau

Abstract

Recent advances have enabled transfer of genes to various types of cells and tissues. The goals of the present study were to transfer genes to nodose sensory neurons using replication-deficient adenovirus vectors and to define the conditions needed to optimize the gene transfer. Neurons were dissociated from rat nodose ganglia and maintained in culture. Cultures were exposed for 30 min to vectors containing the beta-galactosidase gene lacZ driven by either the Rous sarcoma virus (RSV) or the cytomegalovirus (CMV) promoter. Cultures were fixed and treated with X-gal to evaluate lacZ expression 1-7 days after exposure to virus. Increasing concentrations of virus led to dose-related increases in the number of neurons expressing lacZ. LacZ was expressed in 8 +/- 2, 39 +/- 6, and 82 +/- 3% of neurons 1 day after exposure to 10(7), 10(8), and 10(9) pfu/ml of AdRSVlacZ, respectively (P < 0.05). The same doses of AdCMVlacZ led to expression in 41 +/- 9, 60 +/- 10, and 86 +/- 4% of neurons. Expression driven by the CMV promoter was essentially maximal within 1 day and remained stable for at least 7 days. In contrast, expression driven by the RSV promoter was less on day 1 but increased over time (1-7 days). There was no lacZ expression ...Continue Reading

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Citations

Feb 11, 2004·Progress in Biophysics and Molecular Biology·Matthew C Zimmerman, Robin L Davisson
Aug 11, 2001·British Journal of Pharmacology·M J CarrB J Undem
Jul 19, 2001·Clinical and Experimental Pharmacology & Physiology·E C VasquezA K Johnson
Dec 18, 2003·Clinical and Experimental Pharmacology & Physiology·Xiuying MaMark W Chapleau
May 17, 2005·Microbes and Infection·Gregory A AllenMarino De Leon
Jul 19, 2001·Annals of the New York Academy of Sciences·M W ChapleauF M Abboud
Aug 12, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Robin L Davisson

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