PMID: 9639393Jun 25, 1998Paper

Adenovirus-mediated p16 gene transfer prevents drug-induced cell death through G1 arrest in human glioma cells

International Journal of Cancer. Journal International Du Cancer
S HamaN Saijo

Abstract

This study examined the effects of full-length p16 gene transfer by recombinant adenovirus on cell growth and on sensitivity to CDDP or ACNU chemotherapies. We developed a recombinant adenovirus expressing the full-length human p16 gene (AxCA-hp16) by the COS-TPC method. AxCA-hp16 was infected into the p16-null human glioma cell line, U251MG. AxCA-hp16 infection inhibited proliferation of U251MG cells. A proliferation assay employing MTT showed that AxCA-hp16 infection induced chemoresistance, preventing CDDP-induced cell death (11- to 15-fold) and ACNU-induced cell death (80- to 92-fold). In the absence of AxCA-hp16, cell death was induced with CDDP or ACNU at 3 to 5 days after treatment, as demonstrated by Trypan-blue exclusion. Flow-cytometric analysis showed that CDDP or ACNU arrested cells in the G2 phase on day 1 and that cells re-entered the cycle on day 3. However, the cells infected with AxCA-hp16 after CDDP or ACNU treatment showed G1 arrest on day 5 after re-entering the cycle from G2 arrest on day 3. The cells infected with AxCA-hp16 before CDDP or ACNU treatment showed G1 arrest over the 5 days after the infection. This study demonstrated that G1 arrest induced with p16-gene expression prevents ACNU- or CDDP-induce...Continue Reading

References

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Citations

Dec 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Thanh U BarbiePatricia K Donahoe
Jun 1, 2005·Molecular and Cellular Biology·Nicole L CollinsJoan S Brugge
Aug 1, 2009·Cancer Gene Therapy·A P KyritsisJ S Rao
Sep 27, 2000·Neoplasia : an International Journal for Oncology Research·B PucciA Giordano
Dec 31, 2010·International Journal of Cancer. Journal International Du Cancer·Sang Y LeeJames R Connor
Aug 16, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Motoo NaganeWebster K. Cavenee
Apr 3, 1999·Current Opinion in Oncology·K NishioN Saijo
May 15, 2007·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Fumiyuki YamasakiKaoru Kurisu
Dec 31, 1998·Current Opinion in Neurology·J L Darling, T J Warr
Aug 26, 2000·The Journal of Biological Chemistry·T U HaS Maheswaran
Apr 2, 2021·Frontiers in Molecular Neuroscience·Kaushik BanerjeeMaria G Castro

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