Abstract
The tumor suppressor protein p53 participates in normal cell differentiation as well as induction of programmed cell death. The authors investigated the effect of p53 overexpression on spermatogenesis by transferring p53 gene into the rat testes. Replication-deficient recombinant adenovirus vectors were constructed to include cytomegalovirus (CMV) promoter driving wild-type p53 (Ad-CMV-p53) or beta-galactosidase (Ad-CMV-beta-gal). Virus was delivered to cells of the tubules by slow retrograde injection through the rete testis. At 0, 4, 7, and 14 days, testes were removed, weighed, and analyzed histopathologically, including immunohistochemistry for p53, Bcl-2, Bax, and interleukin-1beta converting enzyme (ICE). Testicular weight was decreased in Ad-CMV-p53 group at 14 days after injection, while no change occurred in phosphate-buffered saline-injected controls or Ad-CMV-beta-gal-infected testes. Beyond 4 days, cell degradation in tubules interfered with immunohistochemical observation in the Ad-CMV-p53 group. At 4 days, p53 was expressed mostly in spermatocytes. Bax showed greater expression in the p53 group than in the control or Ad-CMV-beta-gal group. ICE, expressed mostly in spermatids, was more abundant in the p53 group tha...Continue Reading
References
Sep 18, 1992·Cell·L R LivingstoneT D Tlsty
Nov 23, 1992·Cell·M B KastanA J Fornace
Feb 1, 1992·Biology of Reproduction·A A AlcivarN B Hecht
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P ShawJ Costa
Jul 25, 1991·Nature·E Yonish-RouachM Oren
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W E MercerS J Ullrich
Nov 1, 1990·Molecular and Cellular Biology·L DillerB Vogelstein
Jun 30, 1989·Cell·C A FinlayA J Levine
May 31, 1994·Annals of the New York Academy of Sciences·S L Brody, R G Crystal
Jun 1, 1995·Molecular Biotechnology·F L Graham, L Prevec
Jul 29, 1996·International Journal of Cancer. Journal International Du Cancer·M V Blagosklonny, W S el-Deiry
Sep 10, 1996·Human Gene Therapy·S C KoL W Chung
Feb 1, 1997·Biology of Reproduction·K T Blanchard, K Boekelheide
Apr 7, 1997·Biochemical and Biophysical Research Communications·T MuramatsuJ Okumura
May 1, 1997·Archives of Otolaryngology--head & Neck Surgery·E H ChangK F Pirollo
Feb 25, 1998·Biology of Reproduction·Y YinA Morgentaler
Dec 16, 1998·Developmental Biology·Y YinA Morgentaler
Feb 24, 2001·The Journal of Gene Medicine·T ShirakawaS Kamidono
Citations
Oct 25, 2011·PloS One·Wei LiYuan-qiang Zhang
Feb 5, 2008·Yi chuan = Hereditas·Shao-Qin GeShu-Mei Mu
Feb 7, 2012·Medical Hypotheses·Sheng-jie GuoWei Li
Jan 2, 2007·Apoptosis : an International Journal on Programmed Cell Death·Carlos LizamaRicardo D Moreno
Jan 22, 2004·Molecular Reproduction and Development·Rui GuoDaishu Han
Jul 27, 2007·Journal of Molecular Histology·Murat TosunMustafa Cihat Avunduk
Nov 6, 2014·American Journal of Physiology. Endocrinology and Metabolism·Weiwei SunDengshun Miao
Aug 14, 2019·Stem Cell Reports·Xiao GaoLei Li