Adenovirus-mediated transfer of siRNA against PTTG1 inhibits liver cancer cell growth in vitro and in vivo

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Jung Cho-RokDong-Soo Im

Abstract

The pituitary tumor transforming (PTTG) gene family comprises PTTG1, 2, and 3. Forced expression of PTTG1 (securin) induces cellular transformation and promotes tumor development in animal models. PTTG1 is overexpressed in various human cancers. However, the expression and pathogenic implications of the PTTG gene family in hepatocellular carcinoma are largely unknown. Gene silencing using short interfering RNA (siRNA) has become an efficient means to study the functions of genes and has been increasingly used for cancer gene therapy approaches. We report that PTTG1, but not PTTG2 and 3, was highly and frequently expressed in liver cancer tissues from patients and highly in SH-J1, SK-Hep1, and Huh-7 hepatoma cell lines. Adenoviral vector encoding siRNA against PTTG1 (Ad.PTTG1-siRNA) depleted PTTG1 specifically and efficiently in SH-J1 hepatoma cells, which resulted in activation of p53 that led to increased p21 expression and induction of apoptosis. The depletion of PTTG1 in HCT116 colorectal cancer cells exhibited a cytotoxic effect in a p53-dependent manner. Ad.PTTG1-siRNA-mediated cytotoxic effect was dependent on expression levels of PTTG1 and p53 in hepatoma cell lines. Huh-7 hepatoma cells, once transduced with Ad.PTTG1-si...Continue Reading

References

Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B BressacM Ozturk
Nov 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A PuisieuxM Ozturk
Nov 19, 1993·Cell·W S el-DeiryB Vogelstein
Mar 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A M HuiS Hirohashi
Apr 1, 1997·Molecular Endocrinology·L Pei, S Melmed
Jan 30, 1999·Genes & Development·P A Sharp
Feb 18, 1999·The Journal of Clinical Endocrinology and Metabolism·X ZhangS Melmed
Jun 30, 2000·Biochemical and Biophysical Research Communications·W BeerheideP Srivatanakul
May 24, 2001·Cell·P V JallepalliC Lengauer
Nov 16, 2001·Biochemical Society Transactions·M Schuler, D R Green
Mar 1, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jordi Bruix, Josep M Llovet
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Aug 1, 2002·Nature Genetics·Snorri S Thorgeirsson, Joe W Grisham
Aug 2, 2002·The Journal of Biological Chemistry·Jing WenDae-Ghon Kim
Oct 31, 2002·The Journal of Biological Chemistry·Yunli ZhouXun Zhang
Dec 7, 2002·Nature Genetics·Sridhar RamaswamyTodd R Golub
Dec 4, 2003·Cancer Letters·Lina ChristopoulouChris Tyler-Smith
Feb 5, 2004·The Breast : Official Journal of the European Society of Mastology·Christine SolbachManfred Kaufmann
Mar 16, 2004·The Journal of Biological Chemistry·Patricia S KhoQiang Yu
Jul 10, 2004·Molecular Cancer·Tariq Hamid, Sham S Kakar
Jul 30, 2004·Frontiers of Hormone Research·Run Yu, Shlomo Melmed
Oct 29, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Won-Kyung ChoDong-Soo Im
Feb 22, 2005·International Journal of Gastrointestinal Cancer·Rajesh KannangaiMichael Torbenson
Jul 2, 2005·Molecular Cell·Ingo H GorrOlaf Stemmann
Sep 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Suk Woo NamJung Young Lee

❮ Previous
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Citations

Dec 7, 2010·Expert Reviews in Molecular Medicine·Vicki E SmithChristopher J McCabe
Oct 26, 2011·Nature Reviews. Gastroenterology & Hepatology·Yan LiJin-Xuan Hou
Aug 15, 2012·Molekuliarnaia biologiia·K V GlebovaM Iu Skoblov
Nov 10, 2010·The Journal of Clinical Investigation·Cho-Rok JungDong-Soo Im
Dec 5, 2009·BMC Genomics·Siva K Panguluri, Sham S Kakar
Nov 19, 2008·Journal of Ovarian Research·Siva Kumar PanguluriSham S Kakar
Jan 1, 2007·Hereditary Cancer in Clinical Practice·Nikola A BowdenRodney J Scott
Aug 9, 2012·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S Q HuangG Ye
Nov 23, 2011·Molecular Medicine·Wilfried PoschDoris Wilflingseder
Mar 20, 2008·World Journal of Gastroenterology : WJG·Patrick Arbuthnot, Liam-Jed Thompson
May 24, 2014·British Journal of Cancer·H KarraP Kronqvist
Sep 24, 2015·Molecular Medicine Reports·Bing ChenYing Tong
Dec 26, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hong-Xia WangJun Wang
Oct 12, 2007·Molecular and Cellular Endocrinology·D S KimC J McCabe
Jun 19, 2007·The Journal of Surgical Research·Steven W WarmannJörg Fuchs
Aug 2, 2014·Bulletin of Mathematical Biology·E A Sarapata, L G de Pillis
Feb 12, 2009·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Takeshi SudaDexi Liu
Nov 30, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Fabio GrizziMaurizio Chiriva-Internati
Oct 13, 2010·Alimentary Pharmacology & Therapeutics·Y TouchefeuG Vassaux
Jan 21, 2012·Histopathology·Henna KarraPauliina Kronqvist
Nov 17, 2010·The FEBS Journal·Min Suk Shim, Young Jik Kwon
Mar 4, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Francisca Molina-JiménezManuel López-Cabrera
Oct 9, 2015·Journal of Hematology & Oncology·Jacqueline E NollAndrew Cw Zannettino
Jan 1, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Yong-Hua XiaZhong-Wei Tian
Jun 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Enjiao ZhangLi Lu
Aug 13, 2015·BMC Medical Genomics·Yun ZhengYunguang Tong
Apr 17, 2015·Reproductive Biology and Endocrinology : RB&E·Annie GirardMarc-André Sirard
May 6, 2015·Life Sciences·Vyacheslav BukoNatalia Sybirna
Mar 27, 2007·Journal of Biochemistry·Juan A Bernal, Agustín Hernández
Mar 27, 2018·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Alexandros IliadisDimitrios N Kanakis

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