PMID: 11923184Mar 30, 2002Paper

Adenovirus-mediated WGA gene delivery for transsynaptic labeling of mouse olfactory pathways

Chemical Senses
Nanako KinoshitaYoshihiro Yoshihara

Abstract

Detailed knowledge of neuronal connectivity patterns is indispensable for studies of various aspects of brain functions. We previously established a genetic strategy for visualization of multisynaptic neural pathways by expressing wheat germ agglutinin (WGA) transgene under the control of neuron type-specific promoter elements in transgenic mice and Drosophila. In this paper, we have developed a WGA-expressing recombinant adenoviral vector system and applied it for analysis of the olfactory system. When the WGA-expressing adenovirus was infused into a mouse nostril, various types of cells throughout the olfactory epithelium were infected and expressed WGA protein robustly. WGA transgene products in the olfactory sensory neurons were anterogradely transported along their axons to the olfactory bulb and transsynaptically transferred in glomeruli to dendrites of the second-order neurons, mitral and tufted cells. WGA protein was further conveyed via the lateral olfactory tract to the olfactory cortical areas including the anterior olfactory nucleus, olfactory tubercle, piriform cortex and lateral entorhinal cortex. In addition, transsynaptic retrograde labeling was observed in cholinergic neurons in the horizontal limb of diagonal ...Continue Reading

Citations

Aug 28, 2004·Journal of Virology·Samir A KelkarPhilip L Leopold
Aug 18, 2010·The Journal of Clinical Investigation·Masahiko AbematsuKinichi Nakashima
Apr 7, 2010·Expert Opinion on Biological Therapy·Youngbuhm HuhKwang-Soo Kim
Aug 26, 2011·Neuroscience Research·Shymaa E BilasyTohru Kataoka
Dec 6, 2003·The European Journal of Neuroscience·Yoko HannoYoshihiro Yoshihara
Apr 6, 2005·Trends in Neurosciences·Edward M Callaway
Oct 8, 2004·Brain Research. Molecular Brain Research·Kyoko IsoyamaHiroshi Shirasawa
Mar 1, 2006·Neuroscience Letters·Kiyoshi DoiToshio Terashima
Mar 18, 2008·Neuron·Liqun LuoKarel Svoboda
Sep 8, 2010·Molecular and Cellular Neurosciences·Yohei OhashiYasushi Miyashita
Jun 5, 2016·Brain Structure & Function·Sarah LibbrechtVeerle Baekelandt
Sep 30, 2005·The Annals of Otology, Rhinology, and Laryngology·Kiyoshi DoiToshio Terashima

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