PMID: 9525610Apr 3, 1998Paper

Adenovirus preterminal protein binds to the CAD enzyme at active sites of viral DNA replication on the nuclear matrix

Journal of Virology
Peter C Angeletti, Jeffrey A Engler

Abstract

Adenovirus (Ad) replicative complexes form at discrete sites on the nuclear matrix (NM) via an interaction mediated by the precursor of the terminal protein (pTP). The identities of cellular proteins involved in these complexes have remained obscure. We present evidence that pTP binds to a multifunctional pyrimidine biosynthesis enzyme found at replication domains on the NM. Far-Western blotting identified proteins of 150 and 240 kDa that had pTP binding activity. Amino acid sequencing of the 150-kDa band revealed sequence identity to carbamyl phosphate synthetase I (CPS I) and a high degree of homology to the related trifunctional enzyme known as CAD (for carbamyl phosphate synthetase, aspartate transcarbamylase, and dihydroorotase). Western blotting with an antibody directed against CAD detected a 240-kDa band that comigrated with that detected by pTP far-Western blotting. Binding experiments showed that a pTP-CAD complex was immunoprecipitable from cell extracts in which pTP was expressed by a vaccinia virus recombinant. Additionally, in vitro-translated epitope-tagged pTP and CAD were immunoprecipitable as a complex, indicating the occurrence of a protein-protein interaction. Confocal fluorescence microscopy of Ad-infected ...Continue Reading

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Citations

Aug 9, 2003·The Journal of Biological Chemistry·Gemma Serrano-HerasAlicia Bravo
Dec 18, 2008·The Journal of General Virology·W C Russell
Jan 18, 2005·FEMS Microbiology Reviews·Alicia BravoMargarita Salas
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Nov 12, 2019·FEBS Letters·Paloma Hidalgo, Ramón A Gonzalez
Jan 31, 2020·Nucleosides, Nucleotides & Nucleic Acids·Francisco Del Caño-Ochoa, Santiago Ramón-Maiques
Jul 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Chen-Yu Wang, Bill Sugden
Oct 20, 2000·The Journal of General Virology·W C Russell
May 14, 2005·The Journal of Biological Chemistry·Frederic D SigoillotHedeel I Guy
Jul 22, 2021·Protein Science : a Publication of the Protein Society·Francisco Del Caño-Ochoa, Santiago Ramón-Maiques

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