Adenovirus Replication Cycle Disruption from Exposure to Polychromatic Ultraviolet Irradiation

Environmental Science & Technology
Bernardo Vazquez-BravoBenito J Mariñas

Abstract

Polychromatic ultraviolet (UV) light with bandwidth of 20 nm and peak emission centered at 224, 254, or 280 nm (UV224, UV254, and UV280, respectively) were used to inactivate human adenovirus type 2 (HAdV-2). Quantitative polymerase chain reaction (qPCR) and reverse transcriptase qPCR assays were used to elucidate the step in the HAdV-2 replication cycle that was disrupted after UV exposure. UV treatment at any of the wavelengths analyzed did not inhibit association of HAdV-2 to the host cells even after exposure to a fluence (UV dose) that would produce a virus inactivation efficiency, measured by plaque assay, greater than 99.99%. In contrast, UV irradiation at all three peak emissions disrupted early E1A gene transcription and viral DNA replication, but different mechanisms appeared to be dominating such disruptions. UV224seemed to have little effect on the integrity of the viral genome but produced a structural transformation of the viral capsid that may inhibit the delivery of viral genome into the host cell nucleus. On the other hand, UV254and UV280did not affect the integrity of the viral capsid, but the mutations they produced on the viral genome might cause the inhibition of the early gene transcription and DNA replica...Continue Reading

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Citations

Dec 8, 2019·Applied and Environmental Microbiology·Elbashir AraudThanh H Nguyen
Aug 15, 2018·Environmental Science & Technology·Zhong QiaoKrista R Wigginton
May 1, 2019·Accounts of Chemical Research·Karl G LindenVanessa Speight
Dec 10, 2021·Environmental Science & Technology·Nicolas AugsburgerPei-Ying Hong

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