Apr 9, 1998

Adenovirus vector-mediated gene transfer into rat retinal neurons and Müller cells in vitro and in vivo

Neuroscience Letters
M FukuharaJ Kosaka


The ability and efficiency of human type V adenovirus-originated vector with CAG promoter to transfer foreign genes was examined in the rat retina. We introduced the adenovirus vector, AxCALacZ with the reporter gene LacZ (beta-galactosidase gene) into cultured retinal cells, and then injected the vector suspension into the vitreous cavity of the eye. Beta-galactosidase staining was observed in both glial and neuronal cells in vitro and in Müller cells near the injection site in vivo. Adenovirus vectors with CAG promoter are useful and efficient for the expression of foreign genes in retinal cells.

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Mentioned in this Paper

Shuttle Vectors
Gene Transfer Techniques
Entire Retina
Lac Gene
Retinal Diseases
Body Cavities
Gene Expression Regulation, Enzymologic

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