PMID: 9533402Apr 9, 1998Paper

Adenovirus vector-mediated gene transfer into rat retinal neurons and Müller cells in vitro and in vivo

Neuroscience Letters
M FukuharaJ Kosaka

Abstract

The ability and efficiency of human type V adenovirus-originated vector with CAG promoter to transfer foreign genes was examined in the rat retina. We introduced the adenovirus vector, AxCALacZ with the reporter gene LacZ (beta-galactosidase gene) into cultured retinal cells, and then injected the vector suspension into the vitreous cavity of the eye. Beta-galactosidase staining was observed in both glial and neuronal cells in vitro and in Müller cells near the injection site in vivo. Adenovirus vectors with CAG promoter are useful and efficient for the expression of foreign genes in retinal cells.

References

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Citations

Nov 15, 2002·Survey of Ophthalmology·Edward Chaum, Mark P Hatton
Jul 23, 1999·Survey of Ophthalmology·P L KaufmanM Cynader
Jun 9, 2000·Annual Review of Neuroscience·R H Masland, E Raviola
Dec 25, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Siobhan M CashmanRajendra Kumar-Singh
May 17, 2005·Microbes and Infection·Gregory A AllenMarino De Leon

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