Aug 1, 1977

Adenylate cyclase activity in cultured epithelial cells

In Vitro
R M NilesA M Rutenburg


The cyclic AMP metabolism of cultured epithelial cells was investigated. Epinephrine or 1-methyl,3-isobutylxanthine (MIX) alone had no effect on cyclic AMP levels in intact cells, whereas the combination of the two agents yielded a 6- to 10-fold increase in cyclic AMP levels. Both basal and stimulated cyclic AMP levels decreased with increasing cell density. Cell-free adenylate cyclase preparations were stimulated markedly by epinephrine or isoproterenol in the absence of MIX. Since the epithelial cells were found to have a relatively small amount of cyclic nucleotide phosphodiesterase (PDE) activity, the requirement for MIX to visualize intact cell responsiveness to epinephrine could be explained only partially by its PDE inhibitory properties.

  • References9
  • Citations6


Mentioned in this Paper

Squamous Transitional Epithelial Cell Count
Cyclic AMP
Norepinephrine, (+, -)-Isomer
Cyclic Adenosine Monophosphate Measurement
Epinephrine Acetate
Epinephrine Measurement
Cyclic AMP, (R)-Isomer
Adenylyl Cyclase 9

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