Adenylate cyclase activity of mouse sperm during capacitation in vitro: effect of calcium and a GTP analogue

International Journal of Andrology
N J MonksL R Fraser

Abstract

Earlier studies have shown increased adenylate cyclase (AC) activity in epididymal mouse sperm incubated under capacitating conditions in vitro. The present study investigated the effect on AC activity of excluding calcium and/or glucose from the sperm incubation medium, which would modulate expression of fertilizing potential. AC activity was higher in sperm incubated for 120 than 30 min, and was higher in sperm incubated in calcium-containing than calcium-free media for all except acrosome-reacted populations. Calcium added at the time of assay stimulated AC activity, the degree of this response being independent of the functional state of the sperm population. The guanine nucleotide analogue Gpp(NH)p slightly enhanced AC activity, but did not alter the stimulatory effect of calcium. Since calcium can increase AC activity, possibly by interaction with a divalent cation allosteric site on the catalytic subunit of the enzyme, a rise in intracellular calcium levels during capacitation may mediate the increased activity of AC, allowing expression of cAMP dependent events which are a prerequisite for fertilization.

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Citations

Nov 20, 1998·Molecular Reproduction and Development·S A Adeoya-OsiguwaL R Fraser
Aug 1, 1992·Journal of Assisted Reproduction and Genetics·S FuruyaS Suzuki
Apr 28, 2004·Molecular and Cellular Endocrinology·Mark A Baker, R John Aitken
Jun 24, 2000·Proceedings of the Society for Experimental Biology and Medicine·S S KoideM Kamada
Feb 1, 1996·International Journal of Andrology·C EmiliozziP Fénichel
Mar 8, 2005·The Journal of Reproduction and Development·Lynn R FraserOlufunmilayo O Osiguwa
Jan 1, 1996·Free Radical Research·H Zhang, R L Zheng
Oct 1, 2004·Molecular Reproduction and Development·Steve TardifJanice L Bailey
Jul 30, 2014·Biochimica Et Biophysica Acta·Mariano G BuffoneDario Krapf
Aug 1, 1988·Gamete Research·L R Fraser, K K Ahuja
Jul 7, 2007·Archives of Andrology·Melissa L VadnaisGary C Althouse
Oct 17, 2013·The Journal of Biological Chemistry·Eva WertheimerPablo E Visconti
Aug 28, 2020·Molecular and Cellular Endocrinology·Carolina Baro GrafDario Krapf

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