Adenylate cyclase of human fat cells. Expression of epinephrrine-sensitive activation revealed by 5'guanylyl-imidodiphosphate.

The Journal of Clinical Investigation
B CooperR I Gregerman

Abstract

Although catecholamines stimulate lipolysis in human fat cells, activation by epinephrine of adenylate cyclase in human fat cell membranes is not readily observed. The possible role of guanine nucleotides in this reaction has now been examined with human material. Fat cell ghosts were prepared from subcutaneous fat obtained from patients undergoing elective surgery. Adenylate cyclase was assayed with [alpha-32P]ATP as substrate. Fluoride ion stimulated the enzyme 8.3-fold relative to basal levels, but epinephrine activation of cyclase was not statistically significant. GTP did not allow expression of an epinephrine effect. However, the addition of the GTP analogue, 5'-guanylyl-imidodiphosphate [GMP-P(NH)P], along with epinephrine produced 5.7-fold activation of the enzyme (P less than 0.001). GMP-P(NH)P alone was without stimulatory effect. Comparable augmentation by GMP-P (NH) P of adenylate cyclase activity was seen with isoproterenol, norepinephrine, and epinephrine. Propranolol blocked catecholamine-GMP-P (NH) P stimulation of the enzyme, suggesting that the nucleotide-dependent activation of catecholamine-sensitive adenylate cyclase is mediated by beta-receptors. GMP-P(NH)P may prove useful in allowing in vitro demonstrati...Continue Reading

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Citations

Jul 18, 1977·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·H KatherB Simon
Dec 1, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·H Kather, B Simon
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Apr 1, 1977·The Journal of Clinical Investigation·H Kather, B Simon
Jul 1, 1977·Klinische Wochenschrift·H KatherB Simon
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Jul 1, 1981·The American Journal of Physiology·D Baum, P Oyer
Jun 1, 1982·Archives of Biochemistry and Biophysics·T M KellyR I Gregerman
Nov 1, 1977·Clinics in Endocrinology and Metabolism·J B Young, L Landsberg

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