PMID: 1208592Dec 1, 1975Paper

Adenylate cyclase stimulation by trypsin

Proceedings of the Society for Experimental Biology and Medicine
W L RyanG L Curtis

Abstract

Adenylate cyclase activity of a rat embryo fibroblast cell line (F111) is markedly increased by brief treatment with 1:300 trypsin. The degree of stimulation depends upon the length of time the cells are treated and the concentration of trypsin. Crystalline trypsin produced a stimulation similar to that obtained with 1:300 trypsin. Further, the addition of soybean trypsin inhibitor blocked the stimulation of adenylate cyclase by 1:300 trypsin. Trypsin-treated adenylate cyclase responds to PGE1, but there is no increase over that of untreated enzyme. This result and the increase in fluoride-stimulated levels of activity suggest that the trypsin is acting upon the catalytic unit of the enzyme.

Citations

Jul 15, 1977·Experientia·A Guiraud-Simplot, L Colobert
Jul 1, 1983·Molecular and Cellular Endocrinology·J HanouneM L Lacombe
Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J L GarciaA M Municio
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·N D Richert, R J Ryan
Aug 21, 1983·Journal of Theoretical Biology·S Strogatz
Sep 30, 1980·Biochimica Et Biophysica Acta·H van den Bosch

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