Adenylate kinase-independent thiamine triphosphate accumulation under severe energy stress in Escherichia coli.

BMC Microbiology
Tiziana GigliobiancoL Bettendorff

Abstract

Thiamine triphosphate (ThTP) exists in most organisms and might play a role in cellular stress responses. In E. coli, ThTP is accumulated in response to amino acid starvation but the mechanism of its synthesis is still a matter of controversy. It has been suggested that ThTP is synthesized by an ATP-dependent specific thiamine diphosphate kinase. However, it is also known that vertebrate adenylate kinase 1 catalyzes ThTP synthesis at a very low rate and it has been postulated that this enzyme is responsible for ThTP synthesis in vivo. Here we show that bacterial, as vertebrate adenylate kinases are able to catalyze ThTP synthesis, but at a rate more than 106-fold lower than ATP synthesis. This activity is too low to explain the high rate of ThTP accumulation observed in E. coli during amino acid starvation. Moreover, bacteria from the heat-sensitive CV2 strain accumulate high amounts of ThTP (>50% of total thiamine) at 37 degrees C despite complete inactivation of adenylate kinase and a subsequent drop in cellular ATP. These results clearly demonstrate that adenylate kinase is not responsible for ThTP synthesis in vivo. Furthermore, they show that E. coli accumulate large amounts of ThTP under severe energy stress when ATP leve...Continue Reading

References

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Citations

Mar 31, 2015·Ukrainskiĭ biokhimicheskiĭ zhurnal·I K Kolos, A F Makarchikov
Jan 17, 2013·Scientific Reports·Tiziana GigliobiancoLucien Bettendorff
Nov 13, 2009·The Journal of Biological Chemistry·Marjorie GangolfLucien Bettendorff
Mar 5, 2014·Metabolic Brain Disease·Lucien BettendorffPierre Wins
Sep 17, 2019·Biochemistry. Biokhimii︠a︡·V A AleshinV I Bunik
Dec 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marvin MöhlerAndres Jäschke

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Methods Mentioned

BETA
electrophoresis
Assay
PCR

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