Adenylate nucleotide levels and energy charge in Arthrobacter crystallopoietes during growth and starvation

Archives of Microbiology
W T Leps, J C Ensign

Abstract

The adenylate nucleotide concentrations, based on internal water space, were determined in cells of Arthrobacter crystallopoietes during growth and starvation and the energy charge of the cells was calculated. The energy charge of spherical cells rose during the first 10 h of growth, then remained nearly constant for as long as 20 h into the stationary phase. The energy charge of rod-shaped cells rose during the first 4 h of growth, then remained constant during subsequent growth and decreased in the stationary growth phase. Both spherical and rod-shaped cells excreted adenosine monophosphate but not adenosine triphosphate or adenosine diphosphate during starvation. The intracellular energy charge of spherical cells declined during the initial 10 h and then remained constant for 1 week of starvation at a value of 0.78. The intracellular energy charge of rod-shaped cells declined during the first 24 h of starvation, remained constant for the next 80 h, then decreased to a value of 0.73 after a total of 168 h starvation. Both cell forms remained more than 90% viable during this time. Addition of a carbon and energy source to starving cells resulted in an increase in the ATP concentration and as a result the energy charge increase...Continue Reading

References

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Citations

Mar 1, 1990·FEMS Microbiology Reviews·U Wanner, T Egli
Nov 7, 2015·PLoS Computational Biology·Kristin J Adolfsen, Mark P Brynildsen
Aug 3, 2010·PloS One·Aaron P WhiteMichael G Surette
Oct 11, 2014·PloS One·Ildefonso M De la FuenteLuis Martínez
Jun 3, 2016·Microbial Cell Factories·Marc CarnicerStephanie Heux
Aug 1, 2016·BMC Microbiology·Jin-Soo ParkChoon-Soo Jeong
Oct 7, 2004·Biophysical Journal·Liqing WangVassily Hatzimanikatis
Mar 10, 2010·Analytical Biochemistry·Hanna MeyerMichael Lalk

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