Adenylyl cyclase in the heart: an enzymocytochemical and immunocytochemical approach

Microscopy Research and Technique
W Schulze, I B Buchwalow

Abstract

This review provides a discussion of the localization of adenylyl cyclase (AC) in normal mammalian heart tissue employing enzymocytochemistry (detection of the catalytic activity of AC by a metal precipitation technique) and immunocytochemistry (immunolabeling of the enzyme protein with antibodies against AC subtypes). By the metal precipitation technique, AC activity was localized in adult guinea pig cardiomyocytes along the sarcolemma and the T-tubule membranes. This reaction can be enhanced by hormones and guanylyl imidodiphosphate, fluoride, and forskolin. With this technique, no precipitates were detected at the sarcoplasmic reticulum. However, under ischemic conditions, AC activity was also found in the junctional sarcoplasmic reticulum of rat cardiomyocytes. Immunocytochemistry revealed AC in the plasma membrane of rat cardiomyocytes. Detection of AC in the perinuclear space of cardiomyocytes might reflect initiation of synthesis and processing of the enzyme protein. Colocalization of AC with cytoskeleton fibers of non-cardiomyocytes emerging in the cell culture of neonatal rat cardiocytes imply a direct cytoskeletal-AC interaction. Finally, it can be stated that the immunolabeling pattern of AC in cryosections of adult ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·C Londos, J Wolff
Mar 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Slezak, S A Geller
Oct 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N W Revis
Oct 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L S Cutler
Sep 18, 1992·Cell·W J Tang, A G Gilman
Jan 1, 1985·Histochemistry·L Rechardt, H Hervonen
Jan 18, 1985·Biochimica Et Biophysica Acta·J Wolff, G H Cook
Nov 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S L Howell, M Whitfield
Feb 1, 1974·Journal of Molecular and Cellular Cardiology·A M KatzM A Kirchberger
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R C WagnerM W Bitensky
Feb 15, 1973·Biochimica Et Biophysica Acta·P V Sulakhe, N S Dhalla
Jan 1, 1984·International Review of Cytology·G PoeggelJ Weiss
Jan 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L S Cutler, C P Christian
Jan 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Slezak, S A Geller
Apr 1, 1995·Bioscience Reports·A G Gilman
Jan 6, 1995·The Journal of Biological Chemistry·R Taussig, A G Gilman
Jun 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Iyengar
Jul 1, 1995·Protein Engineering·E Wallin, G von Heijne

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Citations

Oct 14, 2006·Circulation Research·Rodolphe FischmeisterGrégoire Vandecasteele
Apr 26, 2008·Journal of Receptor and Signal Transduction Research·Benoit BoivinTerence E Hébert
Sep 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·George VaniotisTerence E Hébert
Oct 19, 2014·Molecular Pharmacology·Yuh-Jiin I JongKaren L O'Malley
Jan 16, 2015·Journal of Cardiovascular Pharmacology·Rhiannon CampdenTerence E Hébert
May 9, 2006·Cellular Signalling·Denis J Dupré, Terence E Hébert

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