Adequate Placental Sampling for the Diagnosis and Characterization of Placental Infection by Zika Virus

Frontiers in Microbiology
Emanuella Meneses VenceslauZika-Unicamp Network

Abstract

The detection of Zika virus (ZIKV) in immunoprivileged anatomical sites, potential sites for viral persistence, may guide the confirmation of undefined cases of ZIKV infection and also bring to light unknown pathways of viral transmission. Thus, this study aimed to characterize ZIKV infection in stratified, standardized placental samples in women with exanthematic febrile manifestations during pregnancy and compare findings to the standard investigation protocol of official health agencies. To this end, a case series of placental findings within a prospective cohort study was conducted over a period of 24 months. Serum/urine were obtained at the time of clinical case identification. Placental sampling was performed following standard investigation protocol (samples of 1.0 cm sent to a reference laboratory) and in a systematic way at various regions, such as chorionic plate, chorionic villi, basal plate, amniotic membrane, and umbilical cord, for subsequent ZIKV identification and quantification. Clinical information was obtained and histological preparation with hematoxylin-eosin staining for morphological evaluation was performed. This case series included 17 placentas systematically collected. Of these, 14 were positive by qR...Continue Reading

References

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Citations

Oct 5, 2020·Placenta·Guilherme M NobregaUNKNOWN Zika-Unicamp Network
Mar 18, 2021·Epidemiologia E Serviços De Saúde : Revista Do Sistema Unico De Saúde Do Brasil·Geraldo DuarteFlor Ernestina Martínez-Espinosa
May 20, 2021·Revista Da Sociedade Brasileira De Medicina Tropical·Geraldo DuarteFlor Ernestina Martinez-Espinosa
Jun 11, 2021·PLoS Neglected Tropical Diseases·Rebeca Costa Castelo BrancoMaria do Desterro Soares Brandão Nascimento
Jun 29, 2021·Revista brasileira de ginecologia e obstetrícia : revista da Federação Brasileira das Sociedades de Ginecologia e Obstetrícia·Guilherme de Moraes NobregaMaria Laura Costa

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Methods Mentioned

BETA
biopsies
PCR

Software Mentioned

OpenStax
GraphPad Prism
GraphPad

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