Adf-1 is a nonmodular transcription factor that contains a TAF-binding Myb-like motif.

Molecular and Cellular Biology
G CutlerR Tjian

Abstract

Adf-1 is an essential Drosophila melanogaster sequence-specific transactivator that binds the promoters of a diverse group of genes. We have performed a comprehensive mapping of the functional domains of Adf-1 to study the role of transactivators in the process of gene activation. Using a series of clustered point mutations and small deletions we have identified regions of Adf-1 required for DNA binding, dimerization, and activation. In contrast to most enhancer-binding factors, the Adf-1 activation regions are nonmodular and depend on an intact protein, including the Adf-1 DNA-binding domain, for activity. Like many transcriptional activators, Adf-1 contains a TFIID-binding domain that can interact with specific TAF subunits. Although TAFs are required for Adf-1-directed activation, TAF binding is not sufficient, suggesting that Adf-1 may direct multiple essential steps during activation. Interestingly, both the TAF-binding domain and the DNA-binding domain contain sequences homologous to those of the Myb family of DNA-binding domains. Thus, Adf-1 has evolved an unusual structure containing two versions of the Myb motif, one that binds DNA and one that binds proteins.

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Citations

Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·T O'Brien, R Tjian
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·M E AndrésG Mandel
Aug 30, 2008·Molecular Biology and Evolution·Shamoni MaheshwariDaniel A Barbash
Jan 27, 2007·Journal of Neurochemistry·Laxminarayana KorutlaScott A Mackler
Dec 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ho-Keun KwonChristophe Benoist
Oct 10, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Gregor ZimmermannMatthew P Scott
Oct 11, 2005·The Journal of Experimental Biology·Bruno ArcàJosé M C Ribeiro
Mar 9, 2007·Development·Jean Z MainesDennis M McKearin
Jun 1, 2002·Gene·Raul Alvarez-Venegas, Zoya Avramova
May 5, 2010·Genes & Development·Haiyun SongKonrad Basler

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