Adherence of Aeromonas caviae to human cell lines Hep-2 and Caco-2

Journal of Medical Microbiology
J P ThornleyA Eley

Abstract

Adherence of Aeromonas caviae to HEp-2 and Caco-2 cell monolayers was investigated with 24 clinical isolates. Growth phase, temperature, multiplicity of infection and length of incubation affected adherence. Treatment of the bacteria with trypsin, sodium metaperiodate, mechanical shearing and the addition of cytochalasin B and cycloheximide to the monolayer significantly reduced the adherence capabilities of the strains investigated. The use of chloramphenicol to inhibit protein synthesis reduced the adhesive capabilities of bacteria grown in liquid medium and those subjected to mechanical shearing. Light microscopy, scanning and transmission electron microscopy were employed in the investigation of bacteria-bacteria and bacteria-monolayer interactions and indicated similarities with the aggregative adherence patterns of the Enterobacteriaceae. The presence of extracellular bacterial appendages and their correlation with increased adhesive capacity may indicate a role in the process of adherence.

Citations

Feb 6, 2004·Pathologie-biologie·C Harf-MonteilH Monteil
Jun 30, 2001·FEMS Microbiology Letters·S MerinoJ M Tomás
Jul 13, 2000·FEMS Immunology and Medical Microbiology·A Kelleher, S M Kirov
Feb 9, 2012·Infection and Immunity·Nahal HadiJonathan G Shaw
Dec 19, 2000·Infection and Immunity·I GryllosJ M Tomás
Dec 31, 2005·Journal of Bacteriology·Rocío CanalsSusana Merino
Jan 24, 2006·Journal of Bacteriology·Rocío CanalsSusana Merino
Jul 20, 2007·Antonie van Leeuwenhoek·Denise de Oliveira ScoarisBenedito P Dias Filho
Apr 3, 2012·Molecular and Cellular Probes·Mar LarrosaMaría Teresa García-Conesa
Dec 18, 2010·The Journal of Infection·Jennifer L Parker, Jonathan G Shaw
Mar 12, 2004·Journal of Applied Microbiology·R C Vàzquez-JuárezF Ascencio
Jul 19, 2012·Journal of Applied Microbiology·A PianettiB Citterio
Aug 23, 2008·Journal of Medical Microbiology·Sanaa Fadel, Adrian Eley
Sep 28, 2007·Letters in Applied Microbiology·C R A CoutoA C Freitas-Almeida
Dec 19, 2006·Journal of Medical Microbiology·Sanaa Fadel, Adrian Eley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.